Changes in Mammalian Chromatin Structure as a Function of Protein-Poly(ADP-Ribosyl)ation by Endonuclease Digestion
Perez-Lamiguerio, Maria A., Changes in Mammalian Chromatin Structure as a Function of Protein-poly(ADP-ribosyl)ation by Endonuclease Digestion. Master of Science (Biochemistry and Molecular Biology), June 2004. 66 pages, 12 illustrations, Bibliography, 45 titles. Mammalian chromatin was exposed to either Deoxyribonuclease I or Micrococcal Nuclease digestion as a function of time of incubation and enzyme concentration. Endonuclease enzymatic reactions were stopped with EDTA. Samples were run in 1.5% agarose gels and the oligonucleosomal electrophoretic migration patterns compared. Endonuclease experiments were carried out with rat liver chromatin pre-incubated in the presence or absence of 200 μM βNAD+. A solution of 1.0 mM benzamide was used to stop enzymatic modification. The electrophoretic observations demonstrated a faster and increased degradation of chromatin when proteins were poly(ADP-ribosyl)ated prior to digestion. These results support the hypothesis that that the covalent poly(ADP-ribosyl)ation of chromatin proteins, particularly histones, induces a more relaxed structure, rendering chromatin more sensitive to endonuclease digestion.
Cell Anatomy, Cell and Developmental Biology, Cell Biology, Cellular and Molecular Physiology, Computational Biology, Genetics, Genetics and Genomics, Genomics, Life Sciences, Medical Cell Biology, Medical Genetics, Medicine and Health Sciences, Molecular Genetics, Other Cell and Developmental Biology, Other Genetics and Genomics, Mammalian chromatin structure, function, poly(ADP-ribosyl)ation, endonuclease digestion, deoxyribonuclease, micrococcal nuclease digestion, enzymatic reactions, EDTA, rat liver chromatin, proteins, histones
Networked Digital Library of Theses and Dissertations