Identification et caractérisation des G-quadruplexes dans le transcriptome humain

Guanine-rich RNA sequences can fold into a very stable non-canonical tetrahelical structure called G-quadruplex (G4). In RNA, these structures can be associated with several of post-transcriptional roles such as translation, splicing and polyadenylation. However, the assessment and study of G4 structural contributions at the RNA level remains in its early days. The first step in solving this question is to accurately predict the location of G4 in the transcriptome. In this direction, we have developed a bioinformatic approach for the identification and analysis of G4 of the human transcriptome. Using gene sequences and transcribed annotation, the G4 prediction was realized using G4RNA Screener, a software using automated learning to classify G4. We were able to detect more than 1 100 000 regions with potential G4 across the human transcriptome corresponding to more than 308 000 unique G4 regions. An analysis of potential G4 regions was performed by type of RNA and by type of location in the RNA. This work leads to a first complete portrait of the amount and location of potential G4 regions in the human transcriptome, allowing to generate hypotheses about their contribution to post-transcriptional regulation.

Name thesis:degree_name

M. Sc.

Level thesis:degree_level

Maîtrise

Discipline thesis:degree_discipline

Biochimie

Grantor dc:publisher

Université de Sherbrooke

Year dc:date.issued

2019

Author dc:creator

• Belhamiti, Sarah

Advisors dc:contributor.advisor

• Perreault, Jean-Pierre
• Ouangraoua, Aïda

• G-quadruplexe
• Structure d’ARN
• Transcriptome
• Bio-informatique
• Expression génique
• G-quadruplex
• RNA structure
• Bioinformatics
• Gene expression

Licence dc:rights.uri

• http://creativecommons.org/licenses/by-nc-nd/2.5/ca/

Language dc:language.iso

fr, en

Handle dc:identifier.uri

http://hdl.handle.net/11143/15608

OAI identifier oai:identifier

oai:usherbrooke.scholaris.ca:11143/15608