{"id":{"repo_id":"sherbrooke","oai_identifier":"oai:usherbrooke.scholaris.ca:11143/15608"},"canonical_url":"https://search.dev.ndltd.org/etd/sherbrooke/oai:usherbrooke.scholaris.ca:11143/15608","repository":{"repo_id":"sherbrooke","name":"Université de Sherbrooke","base_url":"https://usherbrooke.scholaris.ca/server/oai/request"},"display":{"title":"Identification et caractérisation des G-quadruplexes dans le transcriptome humain","abstract":"Guanine-rich RNA sequences can fold into a very stable non-canonical tetrahelical structure called G-quadruplex (G4). In RNA, these structures can be associated with several of post-transcriptional roles such as translation, splicing and polyadenylation. However, the assessment and study of G4 structural contributions at the RNA level remains in its early days. The first step in solving this question is to accurately predict the location of G4 in the transcriptome. In this direction, we have developed a bioinformatic approach for the identification and analysis of G4 of the human transcriptome. Using gene sequences and transcribed annotation, the G4 prediction was realized using G4RNA Screener, a software using automated learning to classify G4. We were able to detect more than 1 100 000 regions with potential G4 across the human transcriptome corresponding to more than 308 000 unique G4 regions. An analysis of potential G4 regions was performed by type of RNA and by type of location in the RNA. This work leads to a first complete portrait of the amount and location of potential G4 regions in the human transcriptome, allowing to generate hypotheses about their contribution to post-transcriptional regulation.","abstract_html":"Guanine-rich RNA sequences can fold into a very stable non-canonical tetrahelical structure called G-quadruplex (G4). In RNA, these structures can be associated with several of post-transcriptional roles such as translation, splicing and polyadenylation. However, the assessment and study of G4 structural contributions at the RNA level remains in its early days. The first step in solving this question is to accurately predict the location of G4 in the transcriptome. In this direction, we have developed a bioinformatic approach for the identification and analysis of G4 of the human transcriptome. Using gene sequences and transcribed annotation, the G4 prediction was realized using G4RNA Screener, a software using automated learning to classify G4. We were able to detect more than 1 100 000 regions with potential G4 across the human transcriptome corresponding to more than 308 000 unique G4 regions. An analysis of potential G4 regions was performed by type of RNA and by type of location in the RNA. This work leads to a first complete portrait of the amount and location of potential G4 regions in the human transcriptome, allowing to generate hypotheses about their contribution to post-transcriptional regulation.","abstract_has_math":false,"creators":["Belhamiti, Sarah"],"institution":"Université de Sherbrooke","degree_name":"M. Sc.","degree_level":"Maîtrise","degree_discipline":"Biochimie","degree_department":null,"school":null,"contributors":[],"advisors":["Perreault, Jean-Pierre","Ouangraoua, Aïda"],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019","date_published":"2019","updated_at":"2026-07-27T21:07:28Z","subjects":["G-quadruplexe","Structure d’ARN","Transcriptome","Bio-informatique","Expression génique","G-quadruplex","RNA structure","Bioinformatics","Gene expression"],"languages":["fr","en"],"rights":[],"rights_urls":["http://creativecommons.org/licenses/by-nc-nd/2.5/ca/"],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11143/15608","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Perreault, Jean-Pierre","Ouangraoua, Aïda"]},{"key":"dc:creator","label":"Author","values":["Belhamiti, Sarah"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2019-06-20T15:33:29Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2019-06-20T15:33:29Z"]},{"key":"dc:date.issued","label":"Date","values":["2019"]},{"key":"dc:publisher","label":"Institution","values":["Université de Sherbrooke"]},{"key":"dc:type","label":"Dc Type","values":["Mémoire de maîtrise"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biochimie"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Maîtrise"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M. 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In RNA, these structures can be associated with several of post-transcriptional roles such as translation, splicing and polyadenylation. However, the assessment and study of G4 structural contributions at the RNA level remains in its early days. The first step in solving this question is to accurately predict the location of G4 in the transcriptome. In this direction, we have developed a bioinformatic approach for the identification and analysis of G4 of the human transcriptome. Using gene sequences and transcribed annotation, the G4 prediction was realized using G4RNA Screener, a software using automated learning to classify G4. We were able to detect more than 1 100 000 regions with potential G4 across the human transcriptome corresponding to more than 308 000 unique G4 regions. An analysis of potential G4 regions was performed by type of RNA and by type of location in the RNA. This work leads to a first complete portrait of the amount and location of potential G4 regions in the human transcriptome, allowing to generate hypotheses about their contribution to post-transcriptional regulation.","Les séquences de nucléotides riches en guanine peuvent se replier en une structure tétrahélicale non-canonique très stable appelée G-quadruplexe (G4). Dans l’ARN, ces structures peuvent être associées à une multitude de rôles post-transcriptionnels tels que la traduction, l’épissage et la polyadénylation. Cependant, l’appréciation et l’étude des contributions des structures G4 au niveau de l’ARN demeurent à ses balbutiements. La première étape vers la résolution de cette question est de déterminer avec précision la localisation des G4 dans le transcriptome. Dans cette direction, nous avons développé une approche bio-informatique pour l’identification et l’analyse des G4 du transcriptome humain. À partir des séquences géniques et leur annotation en transcrits, la prédiction de G4 a été réalisée à l’aide de G4RNA screener, un outil utilisant l’apprentissage automatisé pour classifier les G4. Nous avons pu détecter plus de 1 100 000 régions avec des potentiels G4 à travers le transcriptome humain correspondant à un peu plus de 308 000 régions uniques de G4. Une analyse des régions G4 potentielles a été faite par type d’ARN et par type de localisation dans l’ARN. Ce travail conduit à un premier portrait complet de la quantité et la localisation des régions G4 potentielles dans le transcriptome humain permettant de générer des hypothèses sur leur contribution à la régulation post-transcriptionnelle."]},{"key":"dc:title","label":"Title","values":["Identification et caractérisation des G-quadruplexes dans le transcriptome humain"]}]}],"canonical_facts":{"dc:contributor.advisor":["Perreault, Jean-Pierre","Ouangraoua, Aïda"],"dc:creator":["Belhamiti, Sarah"],"dc:date.accessioned":["2019-06-20T15:33:29Z"],"dc:date.available":["2019-06-20T15:33:29Z"],"dc:date.issued":["2019"],"dc:description.abstract":["Guanine-rich RNA sequences can fold into a very stable non-canonical tetrahelical structure called G-quadruplex (G4). In RNA, these structures can be associated with several of post-transcriptional roles such as translation, splicing and polyadenylation. However, the assessment and study of G4 structural contributions at the RNA level remains in its early days. The first step in solving this question is to accurately predict the location of G4 in the transcriptome. In this direction, we have developed a bioinformatic approach for the identification and analysis of G4 of the human transcriptome. Using gene sequences and transcribed annotation, the G4 prediction was realized using G4RNA Screener, a software using automated learning to classify G4. We were able to detect more than 1 100 000 regions with potential G4 across the human transcriptome corresponding to more than 308 000 unique G4 regions. An analysis of potential G4 regions was performed by type of RNA and by type of location in the RNA. This work leads to a first complete portrait of the amount and location of potential G4 regions in the human transcriptome, allowing to generate hypotheses about their contribution to post-transcriptional regulation.","Les séquences de nucléotides riches en guanine peuvent se replier en une structure tétrahélicale non-canonique très stable appelée G-quadruplexe (G4). Dans l’ARN, ces structures peuvent être associées à une multitude de rôles post-transcriptionnels tels que la traduction, l’épissage et la polyadénylation. Cependant, l’appréciation et l’étude des contributions des structures G4 au niveau de l’ARN demeurent à ses balbutiements. La première étape vers la résolution de cette question est de déterminer avec précision la localisation des G4 dans le transcriptome. Dans cette direction, nous avons développé une approche bio-informatique pour l’identification et l’analyse des G4 du transcriptome humain. À partir des séquences géniques et leur annotation en transcrits, la prédiction de G4 a été réalisée à l’aide de G4RNA screener, un outil utilisant l’apprentissage automatisé pour classifier les G4. Nous avons pu détecter plus de 1 100 000 régions avec des potentiels G4 à travers le transcriptome humain correspondant à un peu plus de 308 000 régions uniques de G4. Une analyse des régions G4 potentielles a été faite par type d’ARN et par type de localisation dans l’ARN. Ce travail conduit à un premier portrait complet de la quantité et la localisation des régions G4 potentielles dans le transcriptome humain permettant de générer des hypothèses sur leur contribution à la régulation post-transcriptionnelle."],"dc:identifier.uri":["http://hdl.handle.net/11143/15608"],"dc:language.iso":["fr","en"],"dc:publisher":["Université de Sherbrooke"],"dc:rights.uri":["http://creativecommons.org/licenses/by-nc-nd/2.5/ca/"],"dc:subject":["G-quadruplexe","Structure d’ARN","Transcriptome","Bio-informatique","Expression génique","G-quadruplex","RNA structure","Bioinformatics","Gene expression"],"dc:title":["Identification et caractérisation des G-quadruplexes dans le transcriptome humain"],"dc:type":["Mémoire de maîtrise"],"thesis:degree_discipline":["Biochimie"],"thesis:degree_level":["Maîtrise"],"thesis:degree_name":["M. Sc."],"thesis:institution_name":["Faculté de médecine et des sciences de la santé"]},"updated_at":"2026-07-27T21:07:28Z"}