Characterization of Molecular Content, Cytotoxicity, and Anti-Inflammatory Activity of Extractions from Eupatorium serotinum

<p>Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from <em>Arnica montana</em> have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of <em>Eupatorium serotinum</em>, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from <em>E. serotinum.</em> Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.</p>

Name thesis:degree_name

Master of Science - Biology

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2023

Author dc:creator

• Sloan, Jordy

Contributors dc:contributor

• Dr. Robert Wiggers
• Dr. Darrell Fry
• Dr. Karol Chandler-Ezell

• HPLC
• Sesquiterpene lactone
• Eupatorium serotinum
• TNF-alpha
• Inflammation
• Traditional Medicine
• Biology

Repository record dc:identifier

https://scholarworks.sfasu.edu/etds/494

OAI identifier oai:identifier

oai:scholarworks.sfasu.edu:etds-1523