Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from Arnica montana have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of Eupatorium serotinum, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from E. serotinum. Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.
","abstract_html":"<p>Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from <em>Arnica montana</em> have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of <em>Eupatorium serotinum</em>, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from <em>E. serotinum.</em> Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.</p>","abstract_has_math":false,"creators":["Sloan, Jordy"],"institution":null,"degree_name":"Master of Science - Biology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Robert Wiggers","Dr. Darrell Fry","Dr. Karol Chandler-Ezell"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2023,"date_issued":"2023-05-06T07:00:00Z","date_published":"2023-05-06T07:00:00Z","updated_at":"2026-07-24T04:30:37Z","subjects":["HPLC","Sesquiterpene lactone","Eupatorium serotinum","TNF-alpha","Inflammation","Traditional Medicine","Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarworks.sfasu.edu/etds/494","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Robert Wiggers","Dr. Darrell Fry","Dr. Karol Chandler-Ezell"]},{"key":"dc:creator","label":"Author","values":["Sloan, Jordy"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2023-05-01T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science - Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["HPLC","Sesquiterpene lactone","Eupatorium serotinum","TNF-alpha","Inflammation","Traditional Medicine","Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarworks.sfasu.edu/etds/494"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from Arnica montana have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of Eupatorium serotinum, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from E. serotinum. Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.
"]},{"key":"dc:title","label":"Title","values":["Characterization of Molecular Content, Cytotoxicity, and Anti-Inflammatory Activity of Extractions from Eupatorium serotinum"]}]}],"canonical_facts":{"dc:contributor":["Dr. Robert Wiggers","Dr. Darrell Fry","Dr. Karol Chandler-Ezell"],"dc:creator":["Sloan, Jordy"],"dc:date.available":["2023-05-01T07:00:00Z"],"dc:description.abstract":["Inflammation is a complex and varied immune response which can be expressed chronically or as a temporary reaction to foreign invaders. Symptoms of inflammation include swelling, pain, soreness, and difficulty of motion. Recently, the effect of sesquiterpene lactones such as Helenalin from Arnica montana have been studied for their anti-inflammatory properties. In this study, we identified and harvested samples of Eupatorium serotinum, a plant historically used by Native Americans to treat a variety of medical illnesses. Crude extracts of the leaves, flowers, roots, and stems were prepared by ethanol extraction. The cytotoxicity of the different extracts on HEK293 cells was determined by a cell death assay and a cell viability assay. HPLC was performed on the extracts and compared with a Helenalin standard to determine the possible presence of sesquiterpene lactones isolated from E. serotinum. Finally, an inflammation assay was performed on TNF-α stimulated HEK293 cells pretreated with the extract, and the total RNA was extracted and sequenced to study the differential gene expression.
"],"dc:identifier":["https://scholarworks.sfasu.edu/etds/494"],"dc:subject":["HPLC","Sesquiterpene lactone","Eupatorium serotinum","TNF-alpha","Inflammation","Traditional Medicine","Biology"],"dc:title":["Characterization of Molecular Content, Cytotoxicity, and Anti-Inflammatory Activity of Extractions from Eupatorium serotinum"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science - Biology"]},"updated_at":"2026-07-24T04:30:37Z"}