The Overexpression of Basigin-3 in Glioblastoma

<p>Glioblastoma (GBM) is one of the most aggressive forms of brain tumor. With the current standard of care, survival prognosis for GBM patients is 15 months with a five-year survival rate of less than 3%. An increased understanding of the molecular mechanisms leading to cell growth and survival of GBMs may result in novel treatments to target and eradicate the disease. The protein Basigin-2 (aka EMMPRIN) induces the expression of matrix metalloproteinase (MMP) enzymes, and its expression level is positively correlated with GBM tumor grade. In 2011, Liao et al. reported that a splice variant of the basigin gene, called Basigin-3, may have an inhibitory function when bound to Basigin-2 in Human Hepatocellular Carcinoma (HHC) cells, as it decreases tumor growth, invasion and MMP expression. The goal of this study is to determine the effects of Basigin-3 overexpression in GBM cell growth. For this a recombinant fusion protein consisting of Basigin-3 and the red fluorescent protein mKate2 was overexpressed in a GBM cell line (LN229). RT-PCR and RT-qPCR was used to measure MMP gene expression in Basigin-3/mKate2 expressing cells, and confocal microscopy used to confirm over-expression of Basigin-3 in the transfected populations. Our hypothesis that Basigin-3 overexpression would reduce MMP expression cells was not supported by our data suggesting that Basigin-3 in GBM does not as an inhibitor of Basigin-2 function in GBM cells. </p>

Name thesis:degree_name

Master of Science

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2016

Author dc:creator

• Wightman, Samantha M

Contributors dc:contributor

• Robert Belton, Jr., Ph.D.

• GBM
• basigin
• BSG
• glioblastoma
• glioma
• Cancer Biology

Repository record dc:identifier

https://commons.nmu.edu/theses/100

OAI identifier oai:identifier

oai:commons.nmu.edu:theses-1125