Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1457 for “"overexpression"”.
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Targeting Telomerase Overexpression by Synthetic Dosage Lethality
Telomerase reverse transcriptase (hTERT) is upregulated in multiple cancers and accompanied by the evidence suggesting that up-regulation of telomerase expression is linked to tumorigenesis, raised the hypothesis that inhibiting this enzyme is a powerful antitumor strategy. Despite intensive …
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The Overexpression of Basigin-3 in Glioblastoma
… study is to determine the effects of Basigin-3 overexpression in GBM cell growth. For this a recombinant fusion protein consisting of Basigin-3 and the red fluorescent protein mKate2 was overexpressed in a GBM cell line (LN229). RT-PCR and RT-qPCR was used to measure MMP gene expression in …
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The Effects of Tarsh Overexpression on Lung Carcinomas
… to the migration rate of these cell lines, the overexpression of Tarsh decreased migration in A549 cells, but had no effect on H1299 cells. However, the role of p53 in migration is still unclear. The results of this study suggest that the knockdown of p53 decreases cell migration in A549 cells. …
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Constitutive Overexpression of Acyloxyacyl Hydrolase in Mus Musculus
… CD68p-AOAH transgenic mice had constitutive overexpression of AOAH in macrophages, dendritic cells and tissues rich in these cells (liver, spleen and lung). They also secreted the enzyme into blood and deacylated LPS at a faster rate both in vitro and in vivo. Importantly, constitutive …
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Cloning and Overexpression of Yeast Cystathionine γ-Lyase
Cystathionine γ-lyase (CGL) is a protein involved in the conversion of methionine to cysteine as part of the trans-sulfuration pathway. Inactivation of the enzyme, possibly by oxidation of reactive site residues, has been linked to cataractogenesis. This research isolated the cystathionine γ-lyase …
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Consequences of Plk4 overexpression in mouse pancreatic organoids
Overexpression of the polo-like kinase 4 (Plk4), the master regulator of centrosome duplication, leads to centrosome amplification, a common feature of cancer, including pancreatic cancer. Previous studies have shown that overexpression of Plk4 in mice carrying a doxycycline-inducible Plk4 …
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Cell fate reprogramming using transcription factor feedback overexpression
… dynamics and on inefficient transcription factor overexpression. We study a gene regulatory network that determines the cell fate in the hematopoietic lineage and demonstrate that a deterministic model cannot capture the experimentally observed system dynamics. We also propose the use of feedback …
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SIRT6 Overexpression Protects Against Diet-Induced Diabetes in Mice
Numerous studies in a variety of species indicate that caloric restriction (CR) elicits beneficial metabolic effects including reduced adiposity and improved glucose homeostasis, as well as enhanced insulin and leptin sensitivity. Conversely, chronic feeding on high caloric diets brings about …
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Engineering, overexpression, purification and analytical applications of recombinant bioluminescent reporters.
… fusion protein was constructed. This allowed overexpression in E. coli and purification by avidin affinity chromatography. The light emission of purified Gluc was studied at various coelenterazine concentrations to establish the detectability of the new reporter, which was found to reach …
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The effects of catalase and kisspeptin overexpression on amyloid peptide toxicity
… AD brain plus the effects of catalase and KiSS-1 overexpression in neuronal cells were investigated in the present study. Tissue sections from the AD pons region were immunohistochemically stained to determine if catalase or KP colocalized with Aβ deposits. The immunohistochemistry results show …
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Effects of the OTX2ECR2 enhancer & PROX1 overexpression in murine retina development
<p>There is a large subset of retinal progenitor cells that upregulate the <em>OTX2</em> gene in the developing retina of the mouse. OTX2 is a homeobox transcription factor important for the development of photoreceptors and bipolar cells. If OTX2 is knocked out, photoreceptors and bipolar cells …
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Overexpression, purification and characterization of human proapolipoprotein A-I and mutants
The cDNA coding the human proapoA-I was cloned into an Escherichia coli vector, overexpressed and purified to 99% homogeneity and characterized together with apoA-I purified from human plasma. SDS-PAGE, mass spectrometry and Edman sequence analysis showed that the initial Met residue is post …
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AGO2 in overexpression exhibits oncogenic functions KrasG̳1̳2̳D̳ -associated mouse tumor models
… novel oncogenic functions associated with AGO2 overexpression. In short, we have determined that AGO2 overexpression promotes metastasis in an autochthonous mouse model of non-small cell lung cancer while elevated AGO2 levels in B cells contribute to the initiation and maintenance of activated B …
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The Overexpression of a Feedback -Insensitive Anthranilate Synthase in Arabidopsis and Tobacco
Anthranilate synthase (AS) is the controlling enzyme of the Trp biosynthetic pathway. The activity of AS is feedback inhibited by Trp and its analogs such as 5-methyl Trp (5-MT). Trp analogs are toxic to cells because they cause false feedback inhibition of AS and hinder the normal production of …
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Investigation of ACE-overexpression in Myeloid Cell Lines through Whole-Proteome Analysis
… repair. Independently of angiotensin II, ACE overexpression in murine macrophages (ACE 10/10) and neutrophils (NeuACE) has shown remarkable enhanced immune phenotypes with the ability to improve murine survival against B16 melanoma, methicillin-resistant Staphylococcus aureus (MRSA) and …
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Overexpression of Heat Shock Factor 1 Protects against Pathological Proteins in Neurodegenerative Diseases
… also have toxic off-target effects. Furthermore, overexpression of constitutively active HSF1 reduced the accumulation of insoluble TDP-43 in cell culture; however, male mice overexpressing the active form had an infertility phenotype. This thesis reports that overexpression of wild-type HSF1 …
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Reducing cell wall recalcitrance in poplar through overexpression of cell wall degrading enzymes
… in poplar. </p> <p>With current conventional overexpression technologies, detrimental effects from destructive enzymes on plant hosts are still unconquered challenges for generating ideal biomass. In order to utilize transgenic biomass to produce economically competitive lignocellulosic …
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