Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi

Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, I provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1∆ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2013

Author dc:creator

• Lukehart, Joshua Karl

Contributors dc:contributor

• Kyoungtae Kim

• Vps1
• Snc1
• recycling
• early-endosome
• GARP
• Biology

Statement dc:rights

• © Joshua Karl Lukehart

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1309

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2310