{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-2310"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-2310","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi","abstract":"Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, I provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1∆ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables.","abstract_html":"Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, I provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1∆ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables.","abstract_has_math":false,"creators":["Lukehart, Joshua Karl"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Kyoungtae Kim"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-05-01T07:00:00Z","date_published":"2013-05-01T07:00:00Z","updated_at":"2026-07-24T03:16:23Z","subjects":["Vps1","Snc1","recycling","early-endosome","GARP","Biology"],"languages":[],"rights":["© Joshua Karl Lukehart"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/1309","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kyoungtae Kim"]},{"key":"dc:creator","label":"Author","values":["Lukehart, Joshua Karl"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Vps1","Snc1","recycling","early-endosome","GARP","Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Joshua Karl Lukehart"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/1309"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, I provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1∆ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables."]},{"key":"dc:title","label":"Title","values":["Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi"]}]}],"canonical_facts":{"dc:contributor":["Kyoungtae Kim"],"dc:creator":["Lukehart, Joshua Karl"],"dc:description.abstract":["Recycling of cellular membranes and their constituents plays a role for cell survival and growth. In the budding yeast, there are recycling traffics from early and late endosomal compartments to the late Golgi. Here, I examined a possible role for Vps1, a large GTPase, in the recycling traffic of GFP-Snc1 from early endosomes to the late Golgi. In the absence of Vps1, I observed an aberrant accumulation of GFP-Snc1 puncta in the cytoplasm that I identified as early endosomes. The N-terminal GTPase and the C-terminal GED domains of Vps1 are essential for Vps1's function in Snc1 recycling. Our finding of genetic interactions of VPS1 with genes involved in early endosome-to-Golgi traffic further suggests Vps1 functions as a recycling factor in the membrane traffic. Finally, I provide evidence that the severe accumulation of GFP-Snc1 cytoplasmic puncta in vps1∆ cells is attributed to a mild defect in the retention of the GARP component Vps51 at the late Golgi, as well as a severe disruption of actin cables."],"dc:identifier":["https://bearworks.missouristate.edu/theses/1309"],"dc:rights":["© Joshua Karl Lukehart"],"dc:subject":["Vps1","Snc1","recycling","early-endosome","GARP","Biology"],"dc:title":["Vps1, a Novel Recycling Factor For the Traffic of Early Endosome to Late Golgi"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:16:23Z"}