Ενεργοποίηση του ογκογονιδίου ha-ras1 του ανθρώπου από ογκογονίδια ιών και κυττάρων

THE H-RAS1 ONCOGENE IS A MEMBER OF THE RAS ONCOGENE FAMILY. RAS GENES ARE ACTIVATED IN APPROXIMATELY 50% OF HUMAN TUMOURS. THEREFORE, THE UNDERSTANDING OF THEMECHANISMS OF THEIR ACTIVATION IS CONSIDERED TO BE VERY IMPORTANT FOR THE PROGNOSIS, DIAGNOSIS AND THERAPY OF CANCER. THIS THESIS DESCRIBES STUDIES ON THE REGULATION OF THE HUMAN H-RAS1 GENE PROMOTER. THE RESULTS CAN BE SUMMARIZED AS FOLLOWS: THE MUTANT T24 H-RAS1 PROMOTER REGION (FROM A HUMAN BLADDER CARCINOMA) BEHAVES AS A STRONGER PROMOTER THAN THE PROMOTER OF THE NORMAL H-RAS1 GENE. IN CELL LINES WHICH EXPRESS THE MIDDLE T ANTIGEN GENE OF POLYOMA VIRUS BOTH THE NORMAL AND MUTANT T24 H-RAS1 PROMOTERS ARE TRANS-ACTIVATED. IT IS SUGGESTED THAT ATISSUE-SPECIFIC ELEMENT EXISTS IN THE PROMOTER REGION OF THE H-RAS1 GENE AND THAT THE POLYOMA MIDDLE T ANTIGEN TRIGGERS THE EXPRESSION OF PROTEINS THAT TRANS-ACTIVATE THESE PROMOTERS. THE PROMOTER OF THE H-RAS1 GENE RESPOND TO THE PHORBOL ESTER TPA AND CONTAINS MULTIPLE CONSENSUS SEQUENCES WHICH ARE KNOWN TO BE INDUCED BY TPA OR TO BIND TO THE TRANSCRIPTION FACTOR AP-1. IT IS SUGGESTED THAT PROTEINS OF THE AP-1/NUN FAMILY MAY PLAY A ROLE IN THE CONTROL OF THE HUMAN H-RAS1 GENE TRANSCRIPTION . THE ONCOPROTEIN RAS P21 AUTOREGULATED AND ACTS AS A POTENTIAL MEDIATOR OF INSULIN ON THE H-RAS1 GENE PROMOTER. IT IS SUGGESTED THAT THE MUTATED PROTEIN P21 OF THE H-RAS1 GENE MEDIATES THE ACTION OF INSULIN.

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1989

Authors dc:creator

• Pintzas, Alexandros
• Πίντζας, Αλέξανδρος

• TRANS-ΕΝΕΡΓΟΠΟΙΗΣΗ
• ΑΝΤΙΓΟΝΑ ΠΟΛΥΟΜΑ ΙΟΥ
• ΕΠΑΥΞΗΝΤΕΣ ΓΟΝΙΔΙΩΝ
• ΕΣΤΕΡΕΣ ΤΗΣ ΦΟΡΒΟΛΗΣ
• Ινσουλίνη
• Καρκίνος
• Ογκογονίδια
• ΟΓΚΟΠΡΩΤΕΙΝΗ ΤΗΣ Ρ21
• ΠΑΡΑΓΟΝΤΕΣ ΜΕΤΑΓΡΑΦΗΣ
• ΠΡΟΑΓΩΓΕΑΣ (ΥΠΟΚΙΝΗΤΗΣ) ΓΟΝΙΔΙΟΥ
• Enhancers
• Transcription factors
• Trans-activation
• Polyoma virus antigens
• Gene enhancers
• Phorbol esters
• Insulin
• Cancer
• Oncogenes
• Gene promoter
• p21 oncoprotein
• Φυσικές Επιστήμες
• Βιολογία
• Natural Sciences
• Biological Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1307

OAI identifier oai:identifier

oai:10442/1307