Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 19 of 19 for “"Trans-activation"”.
-
Novel Role of ErbB-2 in Inhibition of Jagged-1-Mediated Trans-Activation of Notch in Breast Cancer
… or overexpression of ErbB-2 inhibits Notch-1 transcriptional activity and trastuzumab or lapatinib increased</p><p>Notch-1 transcriptional activity. Furthermore, Notch-1 is a breast oncogene and a novel target for the treatment of trastuzumab resistant ErbB-2 positive breast cancer in vitro. …
-
Insights on the Regulation of the PERIOD 2 Gene in the Cellular Response to DNA Damage
… response and regulates downstream effectors by trans-activation. Recent studies in our lab show that PER2 can bind to p53, and regulates the trans-activation function. This project studied the subcellular distribution of PER2 in response to DNA damage, and explored the role of p53 in the …
-
SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… 19F NMR for characterizing the structure of the trans-activation responsive RNA element of the HIV-2 promoter and human hepatitis B virus encapsidation signal epsilon element.
-
Characterization of a Phosphomimetic Mutant of the ALS Associated Protein TDP-43
<p>Trans-activation response (TAR) DNA-binding protein 43 (TDP-43) is a natively dimeric 414-residue protein that is encoded by the human <em>TARDBP</em> gene that has important implications in the pathogenesis of the neurodegenerative disorders ALS, FTD, and CTE. TDP-43 has been found …
-
Development of pattern recognition technique for classification of RNA secondary structures by small molecules
… unique secondary structures within the Trans-Activation Response element, fluoride riboswitch and pre-queuosine 1 riboswitch could be differentiated based on the position of the fluorophore using the training set clusters. This method provides insight into both the RNA topological and …
-
Investigating luteinizing hormone receptor signal transduction using the cyclic AMP reporter ICUE1
… is usually used to demonstrate LH receptor activation and has historically relied on biochemical methods. ICUEl is an Epac-based cAMP reporter which undergoes conformational changes after binding cAMP. Unlike traditional biochemical assays, ICUEl combined with FRET techniques is capable of …
-
Recombinant Expression, Purification, and Characterization of an HIV-1 Tat-Human Cyclin T1 Chimera
<p>Efficient transcription of the human immunodeficiency virus type 1 (HIV-1) requires the interaction of the viral protein Tat with the trans-activation response (TAR) stem-loop of the long-terminal repeat (LTR) portion of nascent viral RNA. The production of viable transcripts is enhanced …
-
Identification et caractérisation de nouveaux isoformes humains du gène Pax-5
… à cellules B est occupé par le facteur de transcription Pax-5. Afin de pouvoir jouer son rôle lors de l’activation transcriptionnelle, Pax-5 requiert son domaine de liaison à l’ADN situé au bout N-terminal, en plus de son domaine de trans-activation situé au bout C terminal. Nos travaux …
-
Molecular Mechanisms of C11orf95 translocations in cancer.
Highly recurrent chromosome translocations that produce oncogenic fusions are a prime example of practical therapeutic targets translating into clinical successes. We have previously described the genetic driver of supratentorial (forebrain) ependymomas as translocations of the C11orf95 gene of …
-
The Role of EGFR Signaling in Gonadotropin-Induced Steroidogenesis
… a linear pathway whereby LH receptor activation stimulates cAMP production and PKA signaling. Protein Kinase A (PKA) signaling then triggers EGF receptor activation, which activates the Mitogen Activated Protein Kinase (MAPK) cascade to promote steroidogenic acute regulatory (StAR) …
-
Determination of Biomolecular Interdomain Motions using Nuclear Magnetic Resonance
… serve as a molecular mechanism to guide the transitions between orientations. However, our understanding of interdomain dynamics is limited because a useful description of interdomain motions requires an estimate of the probabilities of interdomain conformations, increasing complexity of the …
-
Investigating the aggregation of TDP-43 in models of Amyotrophic Lateral Sclerosis
Trans-Activation Response DNA-binding Protein 43 (TDP-43) is a major constituent of proteinaceous inclusions characteristic of most forms of amyotrophic lateral sclerosis (ALS) and ubiquitin-positive frontotemporal lobar degeneration (FTLD). Normally a nuclear protein, TDP-43 translocates to the …
-
Targeted Inhibition of Sp1 Transcription Factor and Anti-Angiogenesis of Human Pancreatic Cancer
<p>Transcription factor Specificity Protein 1(Sp1) is reported to be essential for vascular endothelial growth factor (VEGF) constitutive expression in human pancreatic adenocarcinoma. The definitive role of Sp1 in angiogenesis, the impact of anti-angiogenic therapy on the Sp1/ VEGF signaling and …
-
Identification and characterisation of KZFPs directly regulated by p53
Approximately 50% of human DNA is composed of transposable elements (TEs), which in subsequent waves colonized our genome across evolutionary history. KRAB zinc finger proteins (KZFPs) are the largest group of DNA binding factors in the human genome and mostly target TEs, controlling chromatin …
-
Identification and characterization of Risp
… proteins. Rev enhances the nucleocytoplasmic transport and the translation of unspliced and single spliced viral mRNAs by binding with high affinity to a specific target element on the HIV-RNA. It was assumed that interaction with cellular factors is essential for Rev function. At the onset of …
-
Cross-modulatory Actions of Cell Cycle Machinery on Estrogen Receptor-alpha Level and Transcriptional Activity in Breast Cancer Cells
… Skp2, in the proteasomal degradation of ERα upon activation by p38MAPK. Interestingly, this regulation of ERα by sequential actions of p38MAPK and Skp2 that was observed specifically during the G1/S transition and S-phases of the cells cycle was found to be critical for the progression of cells …
-
Mechanisms by which natural polyphenols regulate the expression of cytoprotective genes
… insult and reduce oxidative stress. The CNC-bZIP transcription factor, Nrf2, is known to regulate the expression of many of the genes involved in these processes. Under homeostatic conditions, the Nrf2 protein is targeted by its inhibitor Keap1 for ubiquitination in the cytosol. Upon redox stress, …
-
Characterization of signalling cross-talk between the EP2 and FP receptors in endometrial epithelial cells
… from women with fibroids. Real-Time reverse-transcriptase polymerase chain reaction (RT-PCR) revealed that the expression of COX enzymes, EP1, EP2 and EP4 prostanoid receptors and prolactin were not significantly altered while the EP3 subtype receptor was significantly down-regulated in …
-
Ενεργοποίηση του ογκογονιδίου ha-ras1 του ανθρώπου από ογκογονίδια ιών και κυττάρων
… THE UNDERSTANDING OF THEMECHANISMS OF THEIR ACTIVATION IS CONSIDERED TO BE VERY IMPORTANT FOR THE PROGNOSIS, DIAGNOSIS AND THERAPY OF CANCER. THIS THESIS DESCRIBES STUDIES ON THE REGULATION OF THE HUMAN H-RAS1 GENE PROMOTER. THE RESULTS CAN BE SUMMARIZED AS FOLLOWS: THE MUTANT T24 H-RAS1 …