ΚΑΔΜΙΟ ΚΑΙ ΥΔΡΑΡΓΥΡΟΣ ΣΤΟ ΗΠΑΤΟΠΑΓΚΡΕΑΣ ΤΗΣ ΓΑΡΙΔΑΣ CALLIANASSA! TYRRHENA. ΒΙΟΣΥΣΣΩΡΕΥΣΗ ΚΑΙ ΕΠΙΔΡΑΣΕΙΣ ΣΕ ΔΙΑΛΥΤΕΣ ΠΡΩΤΕΙΝΕΣ, ΠΕΠΤΙΔΙΑ ΚΑΙ ΕΝΖΥΜΑ

THE AIM OF THIS RESEARCH EFFORT WAS TO CONTRIBUTE TO THE INVESTIGATION OF THE BIOCHEMICAL CHANGES CAUSED BY HEAVY METALS IN CRUSTANCEANS IN RESPECT TO THE IDENTIFICATION OF MOLECULAR TARGETS OF TOXICITY OF THE ABOVE METALS. SPECIMENS OF THE SEDIMENT DWELLING SHRIMP CALLIANASSA TYRRHENA WERE COLLECTED FROM CLEAN WATERS OF SOUTH EVOIKOS AND EXPOSED IN THE LABORATORY TO DIFFERENT CONCENTRATIONS OF THE HEAVY METALS CADMIUM (CD) AND MERCURY (HG) FOR VARIOUS TIME PERIODS. SINCE HEPOTOPANCREAS IS CONSIDERED AS THE MAJOR DETOXIFICATION ORGAN, EXTRACTS OF THIS TISSUE WERE MAINLY UTILISED IN THIS STUDY. THE EFFECT OF CADMIUM AND MERCURY ON GENERAL PROTEINS, GENERAL PEPTIDES, METAL BINDING PROTEINS AND ON THE ACTIVITY AND MOLECULAR FORMS OF THE ENZYMES GLUTATHIONE S-TRANFERASE, ALKALINE PHOSPHATASE AND ESTERASE WERE STUDIED. TECHNIQUES SUCH AS ELECTROPHORESIS, GEL FILTRATION, ENZYME ACTIVITY ASSAYS, ATOMIC ABSORPTION SPECTROPHOTOMETRY AND REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC), WERE EMPLOYED. CADMIUM AND MERCURY ACCUMULATION WAS FOUND TO CAUSE SIGNIFICANT QUALITATIVE AND QUANTITATIVE CHANGES TO SOLUBLE PROTEIN COMPONENTS IN THE HEPATOPANCREAS OF THE SHRIMP OF C. TYRRENA. SOME OF THESE CHANGES APPEAR TO BE METAL-SPECIFIC WHILE OTHERS COMMON TO BOTH METALS. THE CHANGES WERE SIGNIFICANT FOR BOTH METALS ALTHOUGHMERCURY CONCENTRATIONS IN THE HEPATOPANCREAS WAS 6 TIMES LOWER THAN THAT FOR CADMIUM, IN MOLAR TERMS. THE ABOVE RESULTS SUGGEST THAT FURTHER RESEARCH TOWARDSTHE EFFECT OF ENVIRONMENTALLY SIGNIFICANT METALS ON TISSUE PROTEIN COMPONENTS OF MARINE ORGANISMS MIGHT BE FRUITFUL IN PROVIDING USEFUL INDICATORS FOR EARLY STAGES OF METAL TOXICITY.

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1989

Author dc:creator

• Thaker, Abid

• Cadmium ( Cd )
• CRUSTACEANS
• Enzymes
• Mercury
• Peptides
• Configuration
• Ένζυμα
• Κάδμιο ( Cd )
• Καρκινοειδή
• Πεπτίδια
• Πρωτεΐνες
• Υδράργυρος
• Φυσικές Επιστήμες
• Βιολογία
• Natural Sciences
• Biological Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1301

OAI identifier oai:identifier

oai:10442/1301