Age-related proliferative behavior of mandibular osteoblasts : an in vitro study

Knowledge of age-related changes occurring in the proliferative behavior of osteoblasts is a necessity for orthodontists because the performance of an orthodontic tooth movement requires a sufficient remodeling ability of the affected bony tissue. This could be affected by several local and systemic factors which influence the proliferative activity of the osteoblast with increasing age. As the activity of osteoblasts obtained from sheep are similar to that of human cells, sheep osteoblast cells were obtained to investigate the limit of the dividing function which could be reached by a senescent osteoblast. In an in vitro study, the proliferation rate of 15 sheep from 3 different age-groups was measured using two different proliferation tests (MTT and EZ4U). These depend on the mitochondrial functional activity of the cells and not only on their staining. The difference in cell number and size between the three age-groups was also determined and statistically analyzed using the Friedman-test. The findings of this study indicated a large variance in the proliferative activity between young, middle-aged, and old specimens with a significant standard deviation in each group. However the middle-aged group always showed the relatively highest mean values, which could be reverted to the maturing phase of the cells when the animal had reached the age of puberty. This developmental stage is normally associated with secretion of many hormones and growth factors. The EZ4U-test proved that some of the individual old cells have shown more proliferation potential than others from the young cells, and secondly by MTT-test the proliferation rate on average was higher in the old than in the young group. This means that ageing may have an influence on the proliferative function of the osteoblasts. Further studies on larger samples are needed, in order to verify the frequency of a high proliferative potential in aged osteoblasts.

Author dc:creator

• Juma'a, Mohammad Samer

Contributors dc:contributor

• Jonas, Irmtrud

• Proliferation
• Osteoblasten
• Zellkultur
• ageing
• MTT
• EZ4U
• osteoblasts

Repository record source_url

https://freidok.uni-freiburg.de/data/1630

OAI identifier oai:identifier

oai:freidok.uni-freiburg.de:1630