The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants

Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.

Grantor dc:publisher.institution

Department of Molecular and Cell Biology

Year dc:date.issued

2016

Author dc:creator

• Atkinson, Richard

Advisors dc:contributor.advisor

• Meyers, Ann
• Rybicki, Edward P

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/20474

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/20474