The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants
type: CreativeWork
description: Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.
abstract: Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.
author: Atkinson, Richard
language: eng
date published: 2016
date modified: 2026-07-22T22:23:21Z
provider: University of Cape Town
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Department of Molecular and Cell Biology

PropertyValue
property ID: Handle
value: http://hdl.handle.net/11427/20474