{"id":{"repo_id":"cape-town","oai_identifier":"oai:open.uct.ac.za:11427/20474"},"canonical_url":"https://search.dev.ndltd.org/etd/cape-town/oai:open.uct.ac.za:11427/20474","repository":{"repo_id":"cape-town","name":"University of Cape Town","base_url":"https://open.uct.ac.za/oai/request"},"display":{"title":"The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants","abstract":"Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.","abstract_html":"Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera.","abstract_has_math":false,"creators":["Atkinson, Richard"],"institution":"Department of Molecular and Cell Biology","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":[],"advisors":["Meyers, Ann","Rybicki, Edward P"],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016","date_published":"2016","updated_at":"2026-07-22T22:23:21Z","subjects":[],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/11427/20474","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Meyers, Ann","Rybicki, Edward P"]},{"key":"dc:creator","label":"Author","values":["Atkinson, Richard"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2016-07-20T06:51:44Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2016-07-20T06:51:44Z"]},{"key":"dc:date.issued","label":"Date","values":["2016"]},{"key":"dc:publisher.department","label":"Dc Publisher Department","values":["Department of Molecular and Cell Biology"]},{"key":"dc:publisher.institution","label":"Dc Publisher Institution","values":["University of Cape Town"]},{"key":"dc:type","label":"Dc Type","values":["Master Thesis"]},{"key":"dc:type.qualificationlevel","label":"Dc Type Qualificationlevel","values":["Masters"]},{"key":"dc:type.qualificationname","label":"Dc Type Qualificationname","values":["MSc"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["http://hdl.handle.net/11427/20474"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera."]},{"key":"dc:title","label":"Title","values":["The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants"]}]}],"canonical_facts":{"dc:contributor.advisor":["Meyers, Ann","Rybicki, Edward P"],"dc:creator":["Atkinson, Richard"],"dc:date.accessioned":["2016-07-20T06:51:44Z"],"dc:date.available":["2016-07-20T06:51:44Z"],"dc:date.issued":["2016"],"dc:description.abstract":["Crimean-­Congo Hemorrhagic Fever (CCHF) is a highly infectious, life threatening disease, caused by the Crimean-­Congo Hemorrhagic Fever Virus (CCHFV), a nairovirus that forms part of the Bunyaviridae family. CCHFV has a case fatality rate of approximately 40%. Current diagnostic methods for CCHFV involve the use of live virus antigen, requiring biosafety level 4 (BSL4) conditions for safe handling. The development of a safer diagnostic reagent for detection of this disease is therefore desirable. This project involves the development of a recombinant CCHFV nucleocapsid protein (NP). The nucleocapsid (NP) protein was expressed in Nicotiana benthamiana and purified using a 6x histidine-­tag. The protein was then reacted against serum samples collected from confirmed CCHFV patients to determine its ability to detect IgG antibodies against CCHFV in human sera."],"dc:identifier.uri":["http://hdl.handle.net/11427/20474"],"dc:language.iso":["eng"],"dc:publisher.department":["Department of Molecular and Cell Biology"],"dc:publisher.institution":["University of Cape Town"],"dc:title":["The production of a Crimean-Congo haemorrhagic fever virus diagnostic antigen in plants"],"dc:type":["Master Thesis"],"dc:type.qualificationlevel":["Masters"],"dc:type.qualificationname":["MSc"]},"updated_at":"2026-07-22T22:23:21Z"}