Characterization of Human Airway Epithelial to Mesenchymal Transition upon Rhinovirus Infection

Human rhinovirus (HRV)-induced wheezing illnesses in early life are a major risk factor for subsequent asthma development, and may contribute to induction and progression of airway remodeling (AR) seen in the airways of asthmatic subjects. Thickening of the lamina reticularis is a component of AR that is due to increased deposition of matrix proteins by mesenchymal cells that are increased in asthmatic airways, although the origin(s) of these cells are unclear. We have shown that two different strains of HRV, alone and in the presence of transforming growth factor-β1, caused phenotypic changes in epithelial cells consistent with the process of epithelial to mesenchymal transition. Viral replication was not essential for phenotypic changes, which appear to be mediated, in part, via activation of mitogen activated protein kinase pathways, and via induction of the transcription factor, SLUG. These data support a role for HRV in the development and progression of AR.

Name thesis:degree_name

Master of Science (MSc)

Discipline thesis:degree_discipline

Medical Science

Grantor dc:publisher.institution

Graduate Studies

Year dc:date.issued

2016

Author dc:creator

• Minor, Danielle Marie

Advisor dc:contributor.advisor

• Proud, David

Committee members dc:contributor.committeemember

• Giembycz, Mark
• Kelly, Margaret

• Biophysics--Medical

Statement dc:rights

• University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission.

Language dc:language.iso

eng

DOI

http://dx.doi.org/10.11575/PRISM/25276

OAI identifier oai:identifier

oai:ucalgary.scholaris.ca:11023/3332