The effects of perinatal choline supplementation on neuroinflammation in the plaque niche of APP-NL-G-F mice

Alzheimer’s Disease (AD) is a chronic neurodegenerative disease commonly characterized by the aggregation and deposition of insoluble amyloid beta plaques throughout the brain, and by an associated neuroinflammatory response to these plaques involving astrocytes and microglia. Choline is an essential nutrient with diverse functional roles that has emerged as a promising candidate for the treatment of AD. Localized plaque regions in the polymorphic layer in the medial dentate gyrus of the hippocampus and in the cortex were examined in 9-month-old APP-NL-G-F knock-in AD model mice to determine the effects of perinatal choline supplementation on astrocytosis and gliosis associated with amyloid beta. The size of ionized calcium-binding adaptor molecule 1 (Iba1)-positive cells and clusters were larger in control diet APPNL-G-F mice, although the number and total area covered by Iba1+ cells/clusters were decreased compared to those of control diet C57BL6/J mice. In comparison, choline supplementation significantly reduced the size of Iba1+ cells/clusters in APPNL-G-F mice. These results suggest that perinatal choline supplementation ameliorates neuroinflammatory processes associated with amyloid plaques in these 9-month-old APPNL-G-F mice, and that dietary supplementation of choline might serve as an effective treatment for AD.

Author dc:creator

• Cohen, Benjamin

Advisors dc:contributor.advisor

• Mellott, Tiffany
• Blusztajn, Jan K.

• Neurosciences
• Alzheimer's disease
• Amyloid beta
• Astrocytes
• Microglia
• Mouse
• Neuroinflammation

Language dc:language.iso

en_US

Handle dc:identifier.uri

https://hdl.handle.net/2144/48119

OAI identifier oai:identifier

oai:open.bu.edu:2144/48119