The role of fibrolast-derived cytokines on melasma

Melasma is developed or aggravated by UV radiation, sex hormone, and genetic predisposing factors, however, the exact mechanism of pathogenesis is not clearly demonstrated. Previous studies have been bringing focus into the pigmentation formed by interaction between keratinocytes and melanocytes. Recently, it was reported that dermal fibroblasts played an important roles on the cutaneous pigmentary disorders. Therefore, we studied the role of fibroblast-derived cytokines on the development of melasma. We collected the specimens of perilesional normal site and lesional site by 2 mm punch biopsy technique on the faces of 50 melasma patients. We performed on the specimens to stain of hematoxylin-eosin, Fontana-Masson, Masson trichrome, Verhoeff-van Gieson and immunohistochemical stain of NKI/beteb, hepatocyte growth factor (HGF), stem cell factor (SCF), tumor necrosis factor(TNF)-α, interleukin(IL)-1α, and c-kit. The results of staining were analyzed by estimation of three dermatologists and computerized image analysis. There were marked epidermal thinning of the lesional sites than those of perilesional normal sites, and solar elastosis at lesional dermis were significantly increased. The expression of HGF and SCF significantly increased at lesional dermis and the expression of c-kit was considerably increased at melanocytes of lesional epidermis. However, TNF-α was slightly decreased and IL-1α increased in expression at epidermal melasma skin, but no significant differences as compared to perilesional normal skin. These results may indicate that the dermal fibroblast-derived cytokines, such as HGF and SCF induced activation of melanocytes and subsequently was brought about the development of the melasma.

Author dc:creator

• 김, 지연

Contributors dc:contributor

• 강, 희영
• 대학원 의학과
• 200324257

• 기미
• 섬유아 세포
• 면역조직화학염색
• melasma
• fibroblast
• immunohistochemistry
• HGF
• SCF
• c-kit

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000238
000000000238

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2319