Expression of Prxiredoxin I, III and Thioredoxin in human lung cancer and the normal lung tissues

Continuous growth stimulation by various growth factors and carcinogens as well as chronic oxidative stress of proliferation and growth may co-exist in many solid tumors, such as lung cancer. The relatively new family of novel antioxidant proteins, peroxiredoxins (Prxs), have been implicated in regulating many cellular processes including cell proliferations, differentiation, and apoptosis. They are also reported to function as an oxidative stress-dependent molecular chaperone for many important cellular proteins. However, real pathophysiologic significance of Prx proteins in diseased states, especially in lung disease has not been sufficiently defined. Therefore, we have conducted a study to investigate the distribution and expression of various Prx isoforms in normal lung tissue, lung cancer, and other lung conditions. Surgically resected lung cancer tissue and it's paired control normal lung were used for the conventional SDS-PAGE under both non-reducing and reducing conditions. Proteomic analysis was performed using 2 dimensional electrophoresis. Additionally, immunohistochemistry of normal mouse lung and the cancer tissue was performed with respective anti-Prx isoforms antibodies. From our study, immunohistochemical staining has shown that the isoforms of Prx I, II, III, and V are predominantly expressed in bronchial and alveolar lining epithelium, as well as in alveolar macrophages of the normal mouse lung. It is also found that isoforms of Prx I, III, and thioredoxin are over expressed in the lung cancer tissues when compared to their paired normal control lungs from the same individual. There was also an increased amount of oxidized form of Prx I and a possible truncated form of Prx III in lung cancer samples when analyzed by 2-dimensional electrophoresis. In addition, a 40 kDa intermediate molecular weight protein band and high molecular weight bands of over 200kDa which were recognized by anti-Prx I antibody were present in the tissue extracts of lung cancer patients and this finding needs further investigations. In conclusion, over expression of Prx I, III, and thioredoxin in lung cancer tissue with their possible chaperoning function may represent an attempt of tumor cells to adapt to their microenvironment in a manner which is advantageous for their survival and proliferation with maintenance of their malignant potentials.

Author dc:creator

• 김, 영선

Contributors dc:contributor

• 황, 성철
• 대학원 의학과
• 104930

• 폐암
• Peroxiredoxins
• Thioredoxin
• Over-expression
• Oxidative stress
• Lung cancer

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000292
000000000292

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2304