단순포진바이러스로 유도된 베체트병 마우스모델에서 CD4+CD25+ 조절 T 세포의 역할

"ABSTRACT TABLE OF CONTENTS LIST OF FIGURE LIST OF TABLES Ⅰ. INTRODUCTION Ⅱ. MATERIALS AND METHODS A. Antibodies and reagents B. Animal experiments C. BD-like symptoms D. Flow cytometry E. Splenocyte culture and generation of Treg cells F. Dendritic cells isolation and culture G. Co-culture of CD4+T cells with DCs H. Isolation of CD4+CD25+T cells I.Adoptive transfer of CD4+CD25+T and CD4+CD25-T cells to BD-like mice J. ELISA K. Reverse transcription PCR (RT-PCR) L. Statistical analysis Ⅲ. RESULTS A. CD4+CD25+ Treg cells in BD-like mice compared to BDN mice B. CD4+CD25+T cells were amplified in primary cultures of spleen tissues C. CD4+T cells from normal, BDN, and BD-like splenocytes were co-cultured with normal dendritic cells from normal mice D. Adoptive transfer with CD4+CD25+T cells up-regulated the frequencies of T cell in BD-like mice E. Change of symptoms in BD-like mice after adoptive transfer F. The change of severity score in transferred with CD4+CD25+T cells to BD-like mice G. CD4+CD25+T cells transfer up-regulated serum IL-10 and TGF-β levels and down-regulated IFN-γ and TNF-α levels H. Serum IL-6 and IL-17 levels were down-regulated after transfer I. The differences of serum cytokine amounts between the average of not transferred BD-like mice and 3x10^5 CD4+CD25+T cells transferred each BD-like mouse Ⅳ. DISCUSSION Ⅴ. CONCLUSION REFERENCES 국문요약 "

Author dc:creator

• 심, 주아

Contributors dc:contributor

• 손, 성향
• 대학원 의생명과학과
• 200724296

• 베체트병

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009672
000000009672

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2254