Serum Specific IgG to Cytokeratin : A Useful Serologic Marker for Toluene Diisocyanate-Induced Asthma

PURPOSE: Although specific IgE and IgG to isocyanate-HSA conjugate has been used to predict specific bronchoprovocation test results, they are not sensitive enough for the diagnosis of patients with toluene diisocyanate (TDI)-induced asthma. We evaluate the clinical significance of specific IgG to three major epithelial cytokeratins (CK)-CK8, CK18, and CK19-in 66 patients with TDI-induced asthma (group Ⅰ), compared with three control groups: 169 asymptomatic exposed subjects (group Ⅱ), 64 patients with allergic asthma (group Ⅲ), and 123 unexposed healthy control subjects (group Ⅳ). METHODS: Serum-specific IgG to human recombinant CKs were measured by ELISA, and ELISA inhibition tests were done. The existence of these antibodies was confirmed by IgG immunoblot analysis. Specific IgE and IgG to TDI-HSA conjugates were measured in the same set of the patients by ELISA. RESULTS: The prevalences of serum specific IgG to CK18 and CK19 in group I were highest (26.2% each), followed by that of CK8 (18.5%) and these were significantly higher than those in the other three groups (p < 0.0001). Results of the ELISA inhibition test showed significant inhibition with addition of each CK in a dose-dependent manner. The sensitivity and positive predictive value of specific IgG to CK18 and CK19 were higher than those of specific IgE and IgG antibodies to TDI-HSA conjugate. No significant associations were found between specific IgGs to three CKs and the prevalence of specific IgG and IgE to TDI-HSA conjugate. CONCLUSION: These results suggest that serum specific IgG to CK18 and CK19 can be used as a serologic marker for identifying patients with TDI-induced asthma among exposed workers.

Author dc:creator

• 예, 영민

Contributors dc:contributor

• 박, 해심
• 대학원 의학과
• 103726

• isocyanate
• asthma
• cytokeratin
• diagnosis
• TDI
• 천식
• 사이토케라틴
• 진단

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000179
000000000179

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1448