The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study

ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28

Author dc:creator

• 이, 현수

Contributors dc:contributor

• 대학원 의학과
• 108545

• 저출력 레이저
• 섬유아세포
• 켈로이드

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000018908
000000018908

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/11893