{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/11893"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/11893","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study","abstract":"ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28","abstract_html":"ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28","abstract_has_math":false,"creators":["이, 현수"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["대학원 의학과","108545"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-11-05T04:55:58Z","date_published":"2015-11-05T04:55:58Z","updated_at":"2026-07-24T00:51:50Z","subjects":["저출력 레이저","섬유아세포","켈로이드"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000018908","000000018908"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000018908","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000018908","code":true},{"text":"000000018908","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/11893","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["대학원 의학과","108545","이, 현수"]},{"key":"dc:creator","label":"Author","values":["이, 현수"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-11-05T04:55:58Z","2015"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["저출력 레이저","섬유아세포","켈로이드"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/11893","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000018908","000000018908"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. 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CONCLUSION 22 REFERENCES 23 국문요약 28","Master"]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study","저출력 레이저가 인체 진피 섬유아세포의 신호전달체계에 미치는 영향"]}]}],"canonical_facts":{"dc:contributor":["대학원 의학과","108545","이, 현수"],"dc:creator":["이, 현수"],"dc:date":["2015-11-05T04:55:58Z","2015"],"dc:description":["ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. 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