The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study
type: CreativeWork
description: ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28
abstract: ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28
author: 이, 현수
language: en
keywords: 저출력 레이저, 섬유아세포, 켈로이드
date published: 2015-11-05T04:55:58Z
date modified: 2026-07-24T00:51:50Z
provider: Ajou University
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: Ajou University