The low-level light therapy affects human dermal fibroblast via TGF-β/smad signaling pathway: in vitro study
ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES iv LIST OF TABLES v I. INTRODUCTION 1 II. MATERIAL AND METHODS 3 A. Isolation and culture of normal and keloid fibroblasts 3 B. Irradiation 3 C. Determination of the fluence of LLLT 3 D. Cell viability assay 5 E. Real-time polymerase chain reaction (PCR) 5 F. Western blot analysis 5 G. Incubation with exogenous TGF-ß1 6 H. Statistical analysis 6 RESULTS 7 A. Cell viability assay 7 B. The effect of LLLT on the expression of mRNA of TGF-ß/smad signaling molecules 9 C. The effect of LLLT on the protein expression of TGF-ß/smad signaling molecules 12 D. Protein expression of collagen type I, TGF-ß1, smad3 and smad7 after incubation with exogenous TGF-ß1 13 IV. DISCUSSION 17 V. CONCLUSION 22 REFERENCES 23 국문요약 28
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