A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway

As a member of imitation switch (ISWI) family in ATP-dependent chromatin remodeling factors, RSF complex consists of SNF2h ATPase and Rsf-1. Although it has been reported that SNF2h ATPase is recruited to DNA damage sites (DSBs) in poly(ADP-ribosyl) polymerase 1 (PARP1)-dependent manner in DNA damage response (DDR), the function of Rsf-1 is still elusive. Here we show that Rsf-1 is recruited to DSBs confirmed by various cellular analyses (immunofluorescent microscope, micro-irradiation and stably integrated reporter system at a single DNA double-strand break). Rsf-1 rapidly accumulated at the DSB sites. Signal of γ H2AX is gradually reduced at 10 minutes after micro-irradiation whereas signals of Rsf-1 and SNF2h are still retained over 30 minutes after DNA damage. Moreover, depletion of Rsf-1 attenuates the recruitment of SNF2h and γ-H2AX. In addition, Rsf-1 accumulation at DNA damage sites requires PARP1 activity through interacting with PHD domain. Finally, we demonstrate that depletion of Rsf-1 diminishes DNA damage checkpoint signals and repair. Thus, these results reveal a new function of chromatin remodeler Rsf-1 in coordinating DNA signaling and repair.

Author dc:creator

• 조, 수진

Contributors dc:contributor

• 조, 혜성
• 대학원 의생명과학과
• 201125007

• DNA damage
• chromatin remodeling factor
• Rsf-1
• PARP1
• DNA damage checkpoint

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016596
000000016596

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/10857