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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"H2AX"”.
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Widespread Phosphorylation of H2AX by Adenovirus Requires Viral Genome Replication and the E1B-55k or E4orf3 Proteins
… high levels of histone 2AX phosphorylation, or γH2AX. Although phosphorylated H2AX is a characteristic marker of double-stranded DNA breaks, this modification was widely distributed throughout the nucleus of infected cells and was coincident with the bulk of cellular DNA. H2AX phosphorylation …
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A rádiófrekvenciás sugárzás biológiai hatásának vizsgálata emlős sejtekben
… történő esetleges változások kimutatására. A H2AX foszforiláció (γ-H2AX) a DNS kétszál törés egyik legkorábban azonosítható jelátviteli lépése a DNS kijavításnak. Indirekt immuno fluoreszcens assay-vel γ-H2AX intenzitásbeli változást vizsgáltunk HELA és HEK sejteken lézer-scanning …
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The effect of UVA irradiation on the DNA damage response in a human keratinocyte cell line
… blot and immunofluorescence studies found that H2AX activation peaked between 1 and 4h following irradiation with 100 kJ m-2 UVA. Chk2 phosphorylation peaked immediately following UVA irradiation. Inhibition of ATM prior to UVA exposure resulted in a temporal decoupling of H2AX and Chk2 …
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T-lymphocyte senescence and hepatitis C virus infection
… telomeres, I utilised the phospho-histone γ-H2AX, a downstream signal from short telomeres. CD8+ T-lymphocytes expressing γ-H2AX had the form and function of cells with end-stage differentiation. γ-H2AX+ cells had a pro-inflammatory cytokine secretion profile with high expression of IFN-γ and …
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Identification of new functions for BRCT domains
… localization of PTIP into 53BP1- and phospho-H2AX (y-H2AX)-containing nuclear foci, a marker of DNA damage. Here we report that the BRCT domains of PTIP show strong binding to a phosphopeptide corresponding to the C-terminus of y-H2AX. In addition, the BRCT domains of MDC1, MCPH1 and BRCA1 …
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Patterns of post-translational histone modifications, chromatin condensation and DNA fragmentation during apoptosis
… zeigen eine Hyperphosphorylierung am Histon H2AX Serin 139 zehn Minuten nach Induktion und eine starke Ubiquitylierung am Histon H2A sechs Stunden nach Induktion. Untersuchungen zur Freisetzung von spezifisch modifizierten Nucleosomen/Histonen in den Kern oder ins Zytoplasma nach …
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Chemical Genetic Studies of Tumor Suppressors in the Developing Zebrafish
… I sought the role of the DNA damage marker H2AX in early zebrafish development. While studying the DNA damage response in cdc25a mutants, I discovered that H2AX is present and cyclic in pre-midblastula transition embryos. This phosphorylation is necessary for synchronization and relies on …
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LET dependence of radiation-induced bystander effects using human prostate tumor cells
… were micronucleus (MN) formation, [gamma]-H2AX double strand break repair foci, and survival fraction. A 1.5-2.0-fold increase in MN formation was observed in both DU-145 and AG01522 bystander cells after either alpha particle or xray irradiation of the DU-145 target cells. A 1.5-fold …
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Cell-Cell Junction Signaling Regulating DNA Double-Strand Break Repair In Breast Cells
… for the DSBR proteins RAD51, MRE11, and γ-H2AX in single mammary epithelial cells. RAD51 is required for most HR, whereas MRE11 and γ-H2AX function in HR as well as DNA damage signaling. Interestingly, ECM signaling up-regulates HR in cells that have “correct” in vivo-like cell-cell …
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A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway
… accumulated at the DSB sites. Signal of γ H2AX is gradually reduced at 10 minutes after micro-irradiation whereas signals of Rsf-1 and SNF2h are still retained over 30 minutes after DNA damage. Moreover, depletion of Rsf-1 attenuates the recruitment of SNF2h and γ-H2AX. In addition, Rsf-1 …
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Activation of the p53 pathway in combination with photon irradiation for the treatment of neurological tumour cells
… using growth assays, flow cytometry, and H2AX foci analysis, respectively. The findings showed that TP53 status strongly influenced cellular responses to treatment. TP53-wild-type cell lines demonstrated clearer growth control following AMG232 treatment, consistent with activation of …
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ATM-Dependent ERK Signaling in Response to DNA Double Strand Breaks
… electrophoresis, accompanied by dose-dependent H2AX phosphorylation at ser-139 and ATM phosphorylation at ser-1981, indicating ATM activation. Furthermore, BrdU photolysis increased phosphorylation of Chk2 (at thr-68) and p53 (at ser-15). p53 phosphorylation was reduced by the ATM inhibitor …
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Identification of Epstein-Barr virus proteins that inhibit the DNA damage response
… that BKRF4 had no effect on the induction of γ-H2AX, but blocked the subsequent step at which histones H2A/H2AX become polyubiquitylated, which in turn inhibited the recruitment of 53BP1. To identify the host interactions of BKRF4, an affinity purification-mass spectrometry experiment was …
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Application of Femtosecond lasers in confocal and scanning tunnelling microscopy
… to the foci. It is applied to a study of y-H2AX foci in radiation dosed cells to assess various properties of y-H2AX foci as a function of radiation. Work on the SPPX-STM apparatus led to the development of a novel high frequency translation stage, allowing a retro-reflector to be …
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Effect of TIS21 on DNA Damage Repair Induced by Etoposide
… induced DNA damage signaling were examined. H2AX foci formation, representative marker for DNA damage, was increased in TIS21-/- mouse embryo fibroblasts (MEFs) compared to wild type MEFs. Foci formation of H2AX after etoposide treatment in LacZ infected Huh7 cells were more retained than …
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Characterization of a Novel 53BP1-Dependent Mechanism that Promotes Non-Homologous End Joining of Deprotected Telomeres by Increasing Chromatin Mobility
… of histones (notably the phosphorylation of H2AX) and the binding of DNA damage response factors (e.g. MDC1, 53BP1). Although several lines of evidence have pointed to a role for some of these factors in DSB repair through non-homologous end-joining (NHEJ), the mechanism of their contribution …
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Novel roles of microtubule-associated tau in radiation-induced brain injury
… which directly interacts with histone 2AX (H2AX), a crucial marker of DNA damage response (DDR). Importantly, H2AX levels significantly decrease after irradiation in the absence of tau, elucidating tau's vital role in neuronal DDR. Loss of tau also results in an increase of eukaryotic …
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Analysis of radiosensitivity in South African cervical and breast cancer patients
… The G0-micronucleus assay (MN assay) and the γ-H2AX assay are two assays used in radiobiology to study DNA DSB and repair. Breast cancer is the leading cancer amongst South African women, with a lifetime risk of 1 in 34. Since most cancer patients in South Africa present with late-stage disease, …
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The Application of a Statistical Model Investigating Reactive Oxygen Species in Premature Ageing Syndromes
… RD and HGPS fibroblasts showed persistent γ-H2AX foci after DNA damage induction with oxidative stress, yet showed improved repair after induction with a direct DNA break inducing agent, etoposide. Proliferative markers indicated a reduced proliferative capacity in premature ageing …
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