Kartenorientierte Isolierung des Rar1 Locus

The Mla12 allele of the barley-Mla locus confers resistance to powdery mildew isolates expressing the matching avirulence gene avrMla12. The Rar1 (Required for Mla12-specified resistance) locus has been found to be part of the Mla12-governed signal transduction pathway. Previous analysis has demonstrated that rar1-mutant alleles abolish not only Mla12 but also other R gene specificities. To elucidate the molecular mechanisms of R-gene mediated plant defence Rar1 was isolated by map-based cloning. The Rar1-cosegregating marker MWG87 was employed for the identification of five barley YAC clones. Genetic mapping of the YACends showed that the corresponding clones cover the Rar1-containing genome segment. Two of the YAC clones were subcloned into BACs and a Rar1-cosegregating interval of 66kb was defined. Comparative sequencing of the two Rar1-mutants revealed mutations in a candidate gene within the 66kb genome segment. These data provide strong evidence that the candidate gene is in fact the Rar1-encoding locus. The Rar1 protein covers 233 amino acids and has two domains (CHORD) with significant intramolecular homology. These domains show also significant homology to zinc-finger motifs and are conserved most eukaryotic organisms.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

1999

Author dc:creator

• Lahaye, Thomas

Contributors dc:contributor

• Kreuzaler, Fritz

• info:eu-repo/classification/ddc/570
• Biowissenschaften, Biologie
• Gerstenmehltau
• Resistenzgen
• Genort
• Genklonierung

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

ger

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56244