Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"map-based cloning"”.
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Map-based cloning of the NIP gene in model legume Medicago truncatula.
… by rhizobia is regulated. Previous genetic mapping placed NIP at the top of linkage group 1 of the M. truncatula genome. A NIP mapping population was established with the purpose of performing fine mapping in the region containing NIP. DNA from two M. truncatula ecotypes A17 and A20 can be …
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Investigations of self-incompatibility (SI) in perennial ryegrass (Lolium perenne L.)
… in L. perenne is controlled by the S and Z loci, mapping to linkage groups 1 and 2, respectively. None of the gene products has been identified so far. Comparative mapping has identified regions on rice chromosomes 5 (R5) and 4 with synteny to regions of L. perenne genome containing the S and Z …
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Molecular Genetics on the Genus Glycine: I. Phylogenetic Study of the Subtribe Glycininae. II. Development of a Universal Soybean Genetic Map
… the last decade various soybean genetic linkage maps have been developed based not only on morphological and biochemical traits but also on molecular traits such as RFLP, RAPD, AFLP, and SSR data. None of these, however, has been associated with specific chromosomes of soybean. Therefore, this …
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Identification of novel components that connect cellulose synthases to the cytoskeleton
… identified pom2 as a cellulose deficient mutant. Map- based cloning revealed that the gene locus of POM2 corresponded to CELLULOSE SYNTHASE INTERACTING 1 (CSI1). Intriguingly, we previously found the CSI1 protein to interact with the putative cytosolic part of the primary cellulose synthases in a …
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Characterization and molecular cloning of sos3: A gene important for salt tolerance and potassium nutrition in higher plants
… in Arabidopsis. The SOS3 gene was cloned by map-based cloning techniques. SOS3 encodes a protein sharing significant sequence similarity with the B subunit of calcineurin from yeast and neuronal calcium sensor from animals. SOS3 contains three putative EF-hand calcium binding domains and a …
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Analyses of a genic male-sterile mutant in soybean
… of the USDA/ARS/ISU soybean molecular genetic map. The availability of linked DNA markers will facilitate the genetic analysis of the male-sterile gene in relation to soybean breeding programs, and will be a starting point for the isolation of the ms gene by map-based cloning. Another gene, …
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"Physiological and Molecular Analysis of Dnd ""Defense, No Death"" Mutants of Arabidopsis"
… no death"" (dnd) mutants were identified based on their failure to produce the HR when inoculated with P. syringae carrying the avirulence gene avrRpt2. The first objective of this study was to characterize the dnd2 mutants in relation to defense. Like the previously characterized dnd1 …
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High-Resolution Mapping of the Region around the Soybean Virus Resistance Genes, Rsv1 and Rpv1
… to SMV and PMV, respectively. A high-resolution map-based cloning approach was undertaken to isolate Rsv1 and Rpv1 from soybean, with hopes of providing insight into this host-pathogen relationship. A mapping population of 1,056 F2 individuals was constructed from the cross of the resistant …
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Identification and characterization of factors involved in DNA demethylation and anti-silencing in Arabidopsis
… DNA demethylation. In the first system, which is based on 35S promoter driven <em>SUC2</em> (sucrose transporter 2) transgene, a mutant (<em>91-1</em>) was isolated and map-based cloning identified the responsible gene as <em>MBD7</em>(methyl-CpG binding protein 7). As important interpreters of …
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Identification of novel components of the zinc homeostasis mechanism in Arabidopsis thaliana
… addition to the zinc hypersensitivity phenotype. Map-based cloning of the mutated gene in IZS 288 lead to the identification of a novel WD-40 gene that is presumed to form a complex with cullin 4 ubiquitin E3 ligases and take part in the selective degradation of substrate proteins via the …
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Regulation of plant innate immunity: the role of protein import and the novel MOS4-associated complex
… to an oomycete pathogen. MOS6, identified by map-based cloning, encodes an alpha-importin subunit, one of 8 found in Arabidopsis, and has a demonstrated role in nucleocytoplasmic partitioning (protein import). Two other genes cloned by others from this screen, MOS3 and MOS7, encode components …
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Nitrogen signalling in Arabidopsis thaliana
… previously. Further selection of TF genes based on the responses of selected candidates to other macronutrients and abiotic stresses allowed to distinguish between TFs regulated (i) specifically by nitrogen (29 genes) (ii) regulated by general macronutrient or by salt and osmotic stress (6 …