{"id":{"repo_id":"aachen","oai_identifier":"oai:publications.rwth-aachen.de:56244"},"canonical_url":"https://search.dev.ndltd.org/etd/aachen/oai:publications.rwth-aachen.de:56244","repository":{"repo_id":"aachen","name":"RWTH Aachen University","base_url":"https://publications.rwth-aachen.de/oai2d"},"display":{"title":"Kartenorientierte Isolierung des Rar1 Locus","abstract":"The Mla12 allele of the barley-Mla locus confers resistance to powdery mildew isolates expressing the matching avirulence gene avrMla12. The Rar1 (Required for Mla12-specified resistance) locus has been found to be part of the Mla12-governed signal transduction pathway. Previous analysis has demonstrated that rar1-mutant alleles abolish not only Mla12 but also other R gene specificities. To elucidate the molecular mechanisms of R-gene mediated plant defence Rar1 was isolated by map-based cloning. The Rar1-cosegregating marker MWG87 was employed for the identification of five barley YAC clones. Genetic mapping of the YACends showed that the corresponding clones cover the Rar1-containing genome segment. Two of the YAC clones were subcloned into BACs and a Rar1-cosegregating interval of 66kb was defined. Comparative sequencing of the two Rar1-mutants revealed mutations in a candidate gene within the 66kb genome segment. These data provide strong evidence that the candidate gene is in fact the Rar1-encoding locus. The Rar1 protein covers 233 amino acids and has two domains (CHORD) with significant intramolecular homology. These domains show also significant homology to zinc-finger motifs and are conserved most eukaryotic organisms.","abstract_html":"The Mla12 allele of the barley-Mla locus confers resistance to powdery mildew isolates expressing the matching avirulence gene avrMla12. The Rar1 (Required for Mla12-specified resistance) locus has been found to be part of the Mla12-governed signal transduction pathway. Previous analysis has demonstrated that rar1-mutant alleles abolish not only Mla12 but also other R gene specificities. To elucidate the molecular mechanisms of R-gene mediated plant defence Rar1 was isolated by map-based cloning. The Rar1-cosegregating marker MWG87 was employed for the identification of five barley YAC clones. Genetic mapping of the YACends showed that the corresponding clones cover the Rar1-containing genome segment. Two of the YAC clones were subcloned into BACs and a Rar1-cosegregating interval of 66kb was defined. Comparative sequencing of the two Rar1-mutants revealed mutations in a candidate gene within the 66kb genome segment. These data provide strong evidence that the candidate gene is in fact the Rar1-encoding locus. The Rar1 protein covers 233 amino acids and has two domains (CHORD) with significant intramolecular homology. These domains show also significant homology to zinc-finger motifs and are conserved most eukaryotic organisms.","abstract_has_math":false,"creators":["Lahaye, Thomas"],"institution":"Publikationsserver der RWTH Aachen University","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Kreuzaler, Fritz"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1999,"date_issued":"1999","date_published":"1999","updated_at":"2026-07-30T19:41:53Z","subjects":["info:eu-repo/classification/ddc/570","Biowissenschaften, Biologie","Gerstenmehltau","Resistenzgen","Genort","Genklonierung"],"languages":["ger"],"rights":["info:eu-repo/semantics/openAccess"],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22"],"render_values":[{"text":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22","href":"https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22","code":true}]}]},"links":{"outbound_url":"https://publications.rwth-aachen.de/record/56244","outbound_label":"Repository record","outbound_source":"dc:identifier"},"source_record":{"url":"https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56244","prefix":"oai_dc"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Kreuzaler, Fritz"]},{"key":"dc:creator","label":"Author","values":["Lahaye, Thomas"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:coverage","label":"Dc Coverage","values":["DE"]},{"key":"dc:date","label":"Dc Date","values":["1999"]},{"key":"dc:publisher","label":"Institution","values":["Publikationsserver der RWTH Aachen University"]},{"key":"dc:relation","label":"Dc Relation","values":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-244"]},{"key":"dc:type","label":"Dc Type","values":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["info:eu-repo/classification/ddc/570","Biowissenschaften, Biologie","Gerstenmehltau","Resistenzgen","Genort","Genklonierung"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["ger"]},{"key":"dc:rights","label":"Dc Rights","values":["info:eu-repo/semantics/openAccess"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://publications.rwth-aachen.de/record/56244","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The Mla12 allele of the barley-Mla locus confers resistance to powdery mildew isolates expressing the matching avirulence gene avrMla12. The Rar1 (Required for Mla12-specified resistance) locus has been found to be part of the Mla12-governed signal transduction pathway. Previous analysis has demonstrated that rar1-mutant alleles abolish not only Mla12 but also other R gene specificities. To elucidate the molecular mechanisms of R-gene mediated plant defence Rar1 was isolated by map-based cloning. The Rar1-cosegregating marker MWG87 was employed for the identification of five barley YAC clones. Genetic mapping of the YACends showed that the corresponding clones cover the Rar1-containing genome segment. Two of the YAC clones were subcloned into BACs and a Rar1-cosegregating interval of 66kb was defined. Comparative sequencing of the two Rar1-mutants revealed mutations in a candidate gene within the 66kb genome segment. These data provide strong evidence that the candidate gene is in fact the Rar1-encoding locus. The Rar1 protein covers 233 amino acids and has two domains (CHORD) with significant intramolecular homology. These domains show also significant homology to zinc-finger motifs and are conserved most eukaryotic organisms."]},{"key":"dc:source","label":"Dc Source","values":["Aachen : Publikationsserver der RWTH Aachen University III, 93 S. : Ill., graph. Darst. (1999). = Aachen, Techn. Hochsch., Diss., 1999"]},{"key":"dc:title","label":"Title","values":["Kartenorientierte Isolierung des Rar1 Locus"]}]}],"canonical_facts":{"dc:contributor":["Kreuzaler, Fritz"],"dc:coverage":["DE"],"dc:creator":["Lahaye, Thomas"],"dc:date":["1999"],"dc:description":["The Mla12 allele of the barley-Mla locus confers resistance to powdery mildew isolates expressing the matching avirulence gene avrMla12. The Rar1 (Required for Mla12-specified resistance) locus has been found to be part of the Mla12-governed signal transduction pathway. Previous analysis has demonstrated that rar1-mutant alleles abolish not only Mla12 but also other R gene specificities. To elucidate the molecular mechanisms of R-gene mediated plant defence Rar1 was isolated by map-based cloning. The Rar1-cosegregating marker MWG87 was employed for the identification of five barley YAC clones. Genetic mapping of the YACends showed that the corresponding clones cover the Rar1-containing genome segment. Two of the YAC clones were subcloned into BACs and a Rar1-cosegregating interval of 66kb was defined. Comparative sequencing of the two Rar1-mutants revealed mutations in a candidate gene within the 66kb genome segment. These data provide strong evidence that the candidate gene is in fact the Rar1-encoding locus. The Rar1 protein covers 233 amino acids and has two domains (CHORD) with significant intramolecular homology. These domains show also significant homology to zinc-finger motifs and are conserved most eukaryotic organisms."],"dc:identifier":["https://publications.rwth-aachen.de/record/56244","https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118362%22"],"dc:language":["ger"],"dc:publisher":["Publikationsserver der RWTH Aachen University"],"dc:relation":["info:eu-repo/semantics/altIdentifier/urn/urn:nbn:de:hbz:82-opus-244"],"dc:rights":["info:eu-repo/semantics/openAccess"],"dc:source":["Aachen : Publikationsserver der RWTH Aachen University III, 93 S. : Ill., graph. Darst. (1999). = Aachen, Techn. Hochsch., Diss., 1999"],"dc:subject":["info:eu-repo/classification/ddc/570","Biowissenschaften, Biologie","Gerstenmehltau","Resistenzgen","Genort","Genklonierung"],"dc:title":["Kartenorientierte Isolierung des Rar1 Locus"],"dc:type":["info:eu-repo/semantics/doctoralThesis","info:eu-repo/semantics/publishedVersion"]},"updated_at":"2026-07-30T19:41:53Z"}