Publikationsserver der RWTH Aachen University
Hyaluronsäure als Trägersubstanz für bioaktive Proteine zur Optimierung der Knochendefektheilung
Abstract
dc:descriptionIn the last few years the progress made in the understanding of bone metabolism and the possibility of producing macromolecules have led to new methods being used in the treatment of bone defects. Basic fibroblast growth factor (100µg), an extremely potent mitogen or bone morphogenetic protein (100µg), whose osteo-inductive effects are well-known, and 1 cm³ hyaluronic acid (Hyalart 10 mg/ml), a component of the extracellular matrix, were combined to form a viscous gel. This composite was developed so that it could be introduced into the most diverse cases of bone defects by means percutaneous injection. In an experimental fracture model a defect of a critical dimensions (diameter 9,4mm, depth 10mm) was performed into the distal femur epiphysis of 25 sheep. In 15 animals the defect was filled either with hyaluronic acid (Ha), Ha+bmp2 or with Ha+bfgf; as a control group the other animals were either treated with autologous bone or the defect was left empty. X-ray and fluorescent photos (12 weeks after the operation) already showed macroscopically clearly that no new bone material had formed. In the control group, however, the results were analysed histo-morphometrically because precisely in the spongiosa plastic group a considerable quantity of bone material had formed. The mean value in the spongiosa plastic cases lay by an increase of 19,97% of new bone material, whereby this reading combines the quantity of implanted bone and newly-formed bone in one figure. In the cases of the empty defects there was an increase of 5,11%. The study shows that composites of Ha+bmp2 or Ha+bfgf are not useful as bone replacment materials. The positive results of the spongiosa plastics confirm the present situation in the treatment of bone defects. Although the autologous spongiosa plastic carries with it a large number of problems (limited supplies, infections, pain, bleeding etc), it can still be looked upon as the GOLDSTANDARD in the treatment of bone defects.
Degree
thesis:*- Grantor dc:publisher
- Publikationsserver der RWTH Aachen University
- Year dc:date
- 2008
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Rehbein, Marcus
- Contributors dc:contributor
-
- Niethard, Fritz Uwe
Subjects
dc:subject × 9Rights
dc:rights- Statement dc:rights
-
- info:eu-repo/semantics/openAccess
- Language dc:language
- ger