Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Cell Separation"”.
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Advanced dielectrophoretic cell separation systems
… investigations into new particle handling and separation methods and techniques. It makes a major contribution to the rapidly expanding field of cell separation technology. A novel dielectrophoretic cell separation system has been developed, which is capable of processing large sample volumes …
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An investigation of centrifugal blood-cell separation
… dissertation investigated the centrifugal, batch separation of whole blood into subpopulations of red blood cells (erythrocytes) and white blood cells (leukocytes). Separations took place in a custom-built centrifuge (using a seal-less, anti-twisting feed/withdrawal system) containing, a 25-ml …
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Selective cell separation utilizing porous immunoaffinity membranes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.
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A microfluidic platform for hematopoietic stem cell separation
… silicon micropost device for hematopoietic stem cell enrichment is discussed, which demonstrates how innovations in microfluidics can revolutionize current clinical therapies. Appropriate surface chemistry for robust immobilization of proteins on the surface of different materials and topography …
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Development of biomimetic microfluidic adhesive substrates for cell separation
Cell separation is important in medical, biological research, clinical therapy, diagnostics and many other areas. The conventional methods of cell sorting have limited applications due to sophisticated equipment settings, high costs, or time-intensive and labor-intensive processing steps. Inspired …
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Cell separation and cryopreservation of cord blood fractions for immunotherapeutic applications
… umbilical cord blood (CB) bank (Anthony Nolan Cell Therapy Centre, ANCTC) in which CB is stored for haematopoietic stem cell (HSC) transplantation. Due to strict quality thresholds, the majority of units are not suitable for transplantation. Therefore, ANCTC aims to create a Biobank allowing …
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High throughput microfluidic technologies for cell separation and single-cell analysis
The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has …
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The Use And Development Of Laser Microdissection To Separate Spermatozoa From Epithelial Cells For Str Analysis
… in the interpretation of mixed genotypes when cell separation of the sexual assailant's sperm from the victim's cells is incomplete. The forensic community continues to seek improvements in cell separation methods from mixtures for DNA typing. This report describes the use of laser …
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Evaluating The Impact of Post-Translational Modifications By The Secreted Zinc Metalloprotease, Gele, On The Major Autolysin of E. Faecalis, Atla, and A Stress-Induced Protein, Salb
… hydrolase of <em>E. faecalis</em> involved in cell separation of dividing cells. SalB is a secreted stress-induced protein regulated by the CroRS system. In addition, these two proteins also appear to be affected by the virulence factor, gelatinase (GelE). GelE is a secreted zinc …
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Isolation and analysis of ribonucleic acids
… acids are concentrated in certain parts of the cell. Separation of these parts usually precedes isolation of the ribonucleic acids they contain. This separation is accomplished by a process of cell fractionation (Mathias, 1966).
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Microfluidic technology for cellular analysis and molecular biotechnology
… for biological analysis of a low number of cells. The technological advances in microfluidics have allowed both forming of valves, mixers and pumps and integrating of optic and electronic components into microfluidic devices to construct complete and functional systems. In this dissertation, …
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Serial protein pairs in cell/surface adhesion
… offers the first systematic investigation of cell/surface adhesion mediated through protein/protein bonds in series. Cell adhesion and migration are facilitated through protein/protein bonds in series established when membrane-bound proteins simultaneously interact with extracellular proteins …
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PEPTIDOGLYCAN REMODELLING FACTORS: KEY PLAYERS IN CELL DIVISION AND HOMEOSTASIS MAINTENANCE OF ESCHERICHIA COLI
… integrity and coordinated growth of the cell envelope are crucial for survival, especially under environmental stress conditions. The lipopolysaccharide-containing outer membrane (OM) acts as a selective barrier against toxic molecules and provides additional mechanical support that …
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Three dimensional optofluidic devices for manipulation of particles and cells
… offer a powerful tool for manipulating single cells noninvasively. Integration of optical functions within microfluidic devices provides a new freedom for manipulating and studying biological samples at the micro scale. In the pursuit to realise such microfluidic devices with integrated optical …
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Novel micro/nanofluidic system for separation and monitoring of cells and proteins in perfusion
… complex biopharmaceutical products based on cells and proteins are transforming modem medicine and advancing treatments for many health conditions. Continuous biomanufacturing is one of the top technology trends in the biopharmaceutical industry to improve biological product quality and …
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Affinity Flow Fractionation for label-free cell sorting
Capture and isolation of flowing cells from body fluids such as peripheral blood, bone marrow or pleural effusion has enormous implications in diagnosis, disease monitoring, and drug testing. However, in many situations the conventional methods of cell sorting are of limited use due to complex …
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The AFM Study of Ovarian Cell Structural Mechanics in the Progression of Cancer
… deciphering the complex structure of individual cells and understanding the symptoms of cancer disease has been a highly emphasized research area. The exact cause of Cancer and the genetic heterogeneity that determines the severity of the disease and its response to treatment has been a great …
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Imprinted Magnetic Traps for Study on Particle Fluctuation, Ordering and Microfluidic Applications
… of functional materials and marker-based cell isolation. During my pursuit of Ph.D. in Physics in Prof. R. Sooryakumar’s lab, I have worked on magnetic trapping platforms based on ferromagnetic thin-film patterns imprinted on a substrate. These design-based platforms serve as excellent …
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Native chromatin extraction and its structure analysis
… real-time qPCR. The total assay time taken for cell separation, chromatin purification and chromatin associated RNAs extraction can be accomplished in less than 2h. Finally, we further applied cryogenic electron microscopy to analyze higher order chromatin structure in near-native state. We …
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Manipulating fluids and fields In multimaterial fibers
… capable of inertial-dielectrophoretic live/dead cell separation at throughputs as high as 100 5[mu]L/min. In addition, we show that UV-transparent hollow fibers can be used as templates to fabricate highly complex 3-D hydrogel microparticles with dielectrophoretic sub-particle localization. …
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