Cell separation and cryopreservation of cord blood fractions for immunotherapeutic applications

In 2008 Anthony Nolan opened the UK’s first public umbilical cord blood (CB) bank (Anthony Nolan Cell Therapy Centre, ANCTC) in which CB is stored for haematopoietic stem cell (HSC) transplantation. Due to strict quality thresholds, the majority of units are not suitable for transplantation. Therefore, ANCTC aims to create a Biobank allowing these units to be stored for other purposes. To ensure cell products retained high levels of viability and potency, this study optimised banking processes starting with assessing the effects of transport conditions.It is essential that units are of the highest possible quality upon arrival at ANCTC, yet there is no consensus as to the optimal transport conditions of HSCs. Therefore, different fresh storage temperatures and the effect of delaying cryopreservation was assessed on three sources of HSCs. Cells were found to be better maintained at refrigerated temperatures and to avoid significant losses in potency, delays in cryopreservation should be minimised to <24 hours for bone marrow and <48 hours for CB and mobilised peripheral blood stem cells. Based upon these observations, ANCTC maintains all fresh samples at refrigerated temperatures and aims to cryopreserve them within 24 hours of collection.

Name dc:type.qualificationname

phd

Level dc:type.qualificationlevel

doctoral

Grantor dc:publisher.institution

Nottingham Trent University

Year dc:date.issued

2014

Author dc:creator

• Fry, LJ

Language dc:language

en