Novel micro/nanofluidic system for separation and monitoring of cells and proteins in perfusion

High-quality complex biopharmaceutical products based on cells and proteins are transforming modem medicine and advancing treatments for many health conditions. Continuous biomanufacturing is one of the top technology trends in the biopharmaceutical industry to improve biological product quality and reduce manufacturing cost. This thesis introduces novel high-throughput microfluidic cell separation and nanofluidic protein quality monitoring technologies. The novel micro/nanofluidic system enables reliable and efficient microfiltration and robust online rapid product quality monitoring during continuous biomanufacturing. This technology overcomes the limitations of the current membrane-based microfiltration and quality monitoring technologies, including filter clogging, low product recovery, manual sample preparation, and off-line analysis. The first part describes the novel cell retention device for perfusion culture based on inertial sorting.

Name thesis:degree_name

Doctoral

Department dc:contributor.department

Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science

Grantor dc:publisher

Massachusetts Institute of Technology

Year dc:date.issued

2019

Author dc:creator

• Kwon, Taehong.

Advisor dc:contributor.advisor

• Jongyoon Han.

• Electrical Engineering and Computer Science.

Statement dc:rights

• MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission.

Licence dc:rights.uri

• http://dspace.mit.edu/handle/1721.1/7582

Language dc:language.iso

eng

Handle dc:identifier.uri

https://hdl.handle.net/1721.1/121723

OAI identifier oai:identifier

oai:dspace.mit.edu:1721.1/121723