Studies on the operator-repressor-effector interactions in the glp regulon of Escherichia Coli K-12

All of the glp genes of Escherichia coli are subject to negative regulation by the glpR-encoded repressor (GlpR). Comparison of the repressor binding affinity for consensus and altered consensus operator regions showed that positions 3, 4, 5, 7 and 8 bp removed from the center of operator symmetry are most important for repressor binding. Cooperative binding of repressors to tandem operators was demonstrated. Cooperativity was maximal when two 20-base pair operators were directly repeated and decreased with the deletion of 2 base pairs or the addition of 4 base pairs between the operators. Cooperative binding was eliminated by a 6 base pair insertion between tandem operators.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biochemistry and Anaerobic Microbiology

Department dc:contributor.department

Biochemistry and Anaerobic Microbiology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1993

Author dc:creator

• Zhao, Ningyue

Chair dc:contributor.committeechair

• Larson, Timothy J.

Committee members dc:contributor.committeemember

• Bender, Patrick K.
• Shirley, Brenda W.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-07112009-040319

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/43667