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Showing 1 to 20 of 385 for “"repressor"”.
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Regulation of the mobile genetic element ICEBs1 by a conserved repressor and anti-repressor
… and transfer by inactivating the ICEBsl repressor protein ImmR. Although ImmR is similar to many characterized phage repressors, we found that, unlike these repressors, inactivation of ImmR requires an ICEBslencoded anti-repressor ImmA (YdcM). Under ICEBsl-inducing conditions, ImmA …
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Revisiting Allostery In Lac Repressor
Lac repressor (LacI) is an allosterically regulated transcription factor which controls expression of the lac operon in bacteria. LacI consists of a DNA-binding domain (DBD) and regulatory domain (RD), connected by a linker called the “hinge”. Binding of a small molecule inducer to the RD relieves …
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Studies on Escherichia coli tryptophan repressor-operator interactions
Trp repressor regulates the expression of TrpEDCBA, aroH, and TrpR operons. Recently, X-ray crystallographic analysis of a Trp repressor-operator complex has suggested a stoichiometry of one repressor dimer to one operator without any direct hydrogen bonds between repressor residues and operator …
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Nuclear receptor co-repressor actions in bladder cancer
… relative mRNA expression of a range NRs and co-repressors in four bladder cancer cell lines. Nuclear co-repressor 1 (NCoR1) is over-expressed in RT-112 (1.6 fold) and EJ-28 cells (2.6 fold). This correlates with reduced sensitivity to NR ligands in EJ-28 cells. Stable over-expression of NCoR1 in …
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Characterization of the repressor from the lambdoid phage HK022.
… to either mode is controlled by the cI and Cro repressors which bind to operators in the λ chromosome and regulate gene expression of adjacent promoters. HK022 is organized similar to λ, but has its own unique repressors and operators which are different from λ. The major goal of this research …
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P22 arc repressor : cooperative DNA binding and transcriptional regulation
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.
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Gene Therapy Using Tet-Repressor System to Modulate Prostate Tumor Microenvironment
<p>Prostate cancer is the most commonly diagnosed malignancy in men in the United States and is projected to be the third most frequent cause of male cancer-related deaths in 2007 after lung and skin cancers. The initial treatment for prostate cancer at early stages is prostatectomy or radiation, …
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Characterization of effect of tryptophan ontrp repressor subunit association using fluorescence spectroscopy
trp repressor is an allosteric protein which regulates the expression of the trp operon in E. coli. Binding of tryptophan to trp repressor causes it to bind with high affinity to trp operator DNA. The active form of the protein is accepted to be dimeric. Preliminary results from our lab and others …
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β-sheet interactions with DNA revealed by the arc repressor-operator complex
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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A genetic and biochemical analysis of LexA repressor cleavage in Escherichia coli K-12.
The LexA repressor of Escherichia coli represses a set of genes that are expressed in response to DNA damage. After inducing treatments, the repressor is inactivated in vivo by a specific cleavage reaction which requires RecA protein. Under physiological conditions in vitro, RecA-dependent cleavage …
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Nuclear receptor co-repressor functions in prostate cancer; in vitro, in vivo and in silico approaches
… proliferative ability. The Nuclear Receptor Co-Repressor 1 (NCOR1) complexes with Histone Deacetylases (HDACs) to repress the action of unliganded NRs, hence, inhibiting their transcriptional and phenotypical effects. NCOR1 was found to be over-expressed in PC-3 cells when compared to …
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Folding Thermodynamic and Kinetic Characterization of Small Model Proteins: Lambda Repressor, U1A, Trpzip2 and PAO -12
Complete prediction of the folding processes for complex protein molecules through first principles is currently not possible (2003). Here we take an empirical approach towards a better understanding of the protein-folding problem, through thermodynamic and kinetic characterizations of several …
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Studies on the operator-repressor-effector interactions in the glp regulon of Escherichia Coli K-12
… to negative regulation by the glpR-encoded repressor (GlpR). Comparison of the repressor binding affinity for consensus and altered consensus operator regions showed that positions 3, 4, 5, 7 and 8 bp removed from the center of operator symmetry are most important for repressor binding. …
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Construction of an <i>Escherichia coli lac</i> Repressor-Based System to Study Human Cytomegalovirus Gene Expression
<p>An <em>Escherichia coli lac</em> repressor-based system was developed to study the roles of human cytomegalovirus (HCMV) genes during viral replication. To this end, a recombinant HCMV expressing the <em>lac</em> repressor was generated (RV<em>lac</em>), and an HCMV-specific promoter was …
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Amyloidogenesis of a Type III-dependent Hpa1 and Structural analysis of a drug-pump repressor DNA complex
… analysis of the Vibrio cholerae VceR drug-pump repressor with bound DNA The VceR protein, from Vibrio cholerae, which is encoded by the vceR gene, represses transcription of the vceCAB operon, which encodes a multidrug efflux pump in this microorganism. This type of pump, composed of a …
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The Use of High Pressure Fluorescence Spectroscopy to Investigate Subunit Interactions in Oligomeric Proteins (Neurophysin, Lac Repressor)
The application of high hydrostatic pressures (1 atm - 3 kbar) has long been known to lead to the dissociation of oligomeric proteins due to the negative volume change accompanying this process. This technique coupled with fluorescence spectroscopy is used in this thesis in order to determine the …
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Selection and Characterization of a Steroid Receptor DNA Binding Domain-Based Repressor of Estrogen Response Element Containing Promoters
To generate a repressor of the ERE-containing genes, we dimerized one of the up-binding mutants, DBD5, with a flexible linker and fused it to a strong KRAB repressor domain. The dimerized FLAGDBD5-KRAB (FdDBD5K) is a much more potent repressor than the monomer FLAGDBD5-KRAB (FDBD5K). The FdDBD5K …
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Estrogen Regulation of Gene Expression and Analysis of the Role of the Coregulator, Repressor of Estrogen Receptor Activity (Rea)
To elucidate the functional activities of REA in diverse estrogen target tissues in vivo, I have used targeted disruption to ablate the REA gene in mice. Genotyping revealed that homozygous animals are not viable, suggesting a crucial role for REA in early development. The viability of heterozygous …
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Repressor of Estrogen Receptor Activity (REA) is a gene dose-dependent coregulator protein affecting estrogen signaling and cell survival
Repressor of Estrogen Receptor Activity (REA) is an evolutionarily conserved protein with established roles in multiple, essential cellular processes including transcription, mitochondrial biogenesis and replicative senescence. Previous reports suggest that REA is a multifunctional protein with …
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