Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii

Nitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from <i>Azotobacter vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing conditions nifF gene expression is stimulated.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Anaerobic Microbiology

Department dc:contributor.department

Anaerobic Microbiology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1989

Author dc:creator

• Bennett, Lisa Tracy

Chair dc:contributor.committeechair

• Dean, Dennis R.

Committee members dc:contributor.committeemember

• Chen, Jiann-Shin
• Larson, Timothy J.
• Johnson, John L.
• Sitz, Thomas O.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-02062013-040043

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/40938