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Showing 1 to 20 of 51 for “"NIF"”.
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Studies on the structure and function of various nif and nif- associated gene products encoded within the Azotobacter vinelandii nif gene cluster
… gene product encoded within the A. vinelandii nif gene cluster. Finally, nucleotide sequence analysis of the regions flanking the nifEN genes revealed the presence of three nif genes (nifT, nifY, and nifX) and four open reading frames (ORF1, ORF2, ORF3, and ORF4). Two of these genes, nifX and …
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Diagnosing inertial confinement fusion implosions at OMEGA and the NIF Using novel neutron spectrometry
… facility and the National Ignition Facility (NIF) to measure the neutron spectra from inertial confinement fusion (ICF) implosions. Using the MRS, the down-scattered neutron (DSn) spectrum has been used to infer the areal density ([rho]R) of ICF implosions for the first time. The DSn technique …
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Shielding design for the time-resolving Magnetic Recoil Spectrometer (MRSt) on the National Ignition Facility (NIF)
Abstract The National Ignition Facility (NIF) is one of the premier inertial confinement fusion (ICF) experiments active today, with the goal of acheiving ignition in a laboratory for the first time. Multiple diagnostics are needed to generate the scientific data necessary for guiding these …
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Exploring evolution of multi-ion effects and electron temperature in ICF implosions at Omega and the NIF
… hotspot electron temperature is not affected significantly by the converging shock and plasma flows like the ion temperature. In this thesis, the design and prototype testing of a new diagnostic for measurements of time-resolved hotspot electron temperature in ignition-relevant implosions at the …
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Mapping nif genes of Rhodobacter capsulatus, and characterization of a mutant with a pleiotropic defect in nitrogen metabolism
… Rhodobacter capsulatus to fix nitrogen (Nif- ) have previously been mapped into six linkage groups (I-VI) by transduction with gene transfer agent (GTA). Because this vector carries only 4600 bp of DNA, linkage can only be demonstrated for short chromosomal distances. In order to …
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Using fusion-product spectroscopy to study inertial fusion implosions, stopping power, and astrophysical nucleosynthesis at OMEGA and the NIF
… Spectrometry capability developed for the NIF has been used to study these protons produced at the 'shock' burn in these implosions, which proceeds the main 'compression' burn by several hundred picoseconds (ps). A 1-D implosion model was developed to interpret the spectra of the emitted …
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Using time-resolved nuclear diagnostics to probe kinetic/multi-ion physics and shock dynamics on OMEGA and the NIF
… implosions. On the National Ignition Facility (NIF) at the Lawrence Livermore National Laboratory (LLNL), implosion dynamics from shock to compression are probed using the magnetic particle time-of-flight (magPTOF) diagnostic. magPTOF is designed to simultaneously measure the shock and …
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Regulation of nitrogen fixation in Klebsiella pneumoniae: NifL, one protein transducing two environmental signals to the nif-transcriptional activator NifA
Klebsiella pneumonia moduliert NifL die Aktivität des Transkriptionsaktivators NifA in Abhängigkeit der Umweltsignale Stickstoff und Sauerstoff. NifL ist ein Flavoprotein, welches das Sauerstoffsignal wahrscheinlich durch einen redoxsensitiven Konformationswechsel transduziert. Wir haben die …
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Using fusion-product spectroscopy to diagnose inertial confinement fusion implosions and study stopping power on OMEGA, the NIF, and Z
… second project at the Nation Ignition Facility (NIF), spectroscopy of secondary nuclear reactions from surrogate deuterium filled implosions are sensitive to hot spot areal density magnitude and asymmetry. Secondary DT neutrons are routinely measured on the NIF using four neutron time of flight …
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Characterization of a major cluster of genes involved in nitrogen fixation and another required for indole-3-acetic acid biosynthesis in the sugarcane endophyte, Acetobacter diazotrophicus
… sequenced, and analyzed the major 31.5 kb nif gene cluster, including both nif and associated genes of A. diazotrophicus. This cluster of 33 genes represents the largest and most complete assembly of contiguous nif/fix and associated genes characterized in any diazotrophic bacterial …
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Development and use of advanced nuclear diagnostics and neural networks to diagnose 3D morphology and power balance in inertial fusion implosions at OMEGA and NIF
… facilities like the National Ignition Facility (NIF) and OMEGA requires new ways of measuring implosion conditions such as the shape of the shell at minimum-volume and the power balance in the hot-spot. This dissertation describes several novel instruments and analysis techniques to measure …
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Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
… the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from <i>Azotobacter vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, …
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Studies of ion kinetic effects in shock-driven inertial confinement fusion implosions at OMEGA and the NIF and magnetic reconnection using laser-produced plasmas at OMEGA
… Ion kinetic effects that are likely to be significant early in ICF implosions, as strong shocks create a high-temperature, moderate-density plasma with an ion mean free path approximately the size of the fuel region, have, for the first time, been explored in a comprehensive experimental …
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Controlled Expression and Functional Analysis of the Iron-Sulfur Cluster Biosynthetic Machinery in Azotobacter vinelandii
… [Fe-S] biosynthetic systems in A. vinelandii, Nif and Isc, can perform overlapping functions. Under normal laboratory growth conditions, no cross-talk between the two systems could be detected. However, elevated expression of Isc components as a consequence of inactivation of the IscR repressor …
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Nitrogen Metabolism and Solvent Production in Clostridium Beijerinckii Nrrl B593
… of C. beijerinckii NRRL B593 that contained the nif genes and ORFs with other putative functions was sequenced in our laboratory. An examination of the nif clusters of C. beijerinckii, C. acetobutylicum and C. pasteurianum revealed apparent differences in the intervening ORFs which suggested …
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Characterization and optimization of signal and background for the time-resolving magnetic recoil spectrometer on the National Ignition Facility
… implosion at the National Ignition Facility (NIF) can be quantified through time-resolved measurements of the neutron spectrum. This information will be obtained with the next-generation Magnetic Recoil Spectrometer (MRSt) that will measure the neutron spectrum (12-16 MeV) with high accuracy …
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Regulation of nitrogen fixation in <i>Klebsiella pneumoniae</i>: Nitrogen and oxygen signal perception by the negative regulator NifL
… Organismus Klebsiella pneumoniae wird der nif - spezifische Transkriptionsaktivator NifA als Reaktion auf molekularen Sauerstoff und Ammonium durch NifL gehemmt. Die Komplexbildung zwischen NifL und NifA (etwa 1:1-Verhältnis) erfolgt, wenn die Synthese der beiden Proteine in Gegenwart von …
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A study of the Rayleigh-Taylor Instability during deceleration in inertial confinement fusion relevant conditions
… on Omega and the Na- tional Ignition Facility (NIF) is necessary to study deceleration-stage RTI experimentally and validate computational modeling. A comprehensive exploration of potential experimen- tal designs on Omega, Omega-EP, and NIF are explored to identify a platform with which …
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Site-directed mutagenesis of the nitrogenase MoFe protein from Azotobacter vinelandii
… protein, and the FeMoco biosynthetic proteins, NifE and NifN, four conserved residues (Cys62, His83, Cys88, Cys154 all proposed P-cluster ligands) within the α- subunit were targeted for site-directed mutagencsis studies. In order to define a range of acceptable substitutions, 35 specific …
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Untersuchung der Expression und Aktivität des Molybdän-Eisen Protein in Wolinella succinogenes
… nur wenige Organismen dafür bekannt, dass sie nif Gene besitzen. Überraschend wurden solche Gene in einem Bakterium aus dem Pansen von Kühen, Wolinella succinogenes, gefunden. Hauptziel dieses Promotionsprojekts war die Nitrogenase von W. succinogenes, die in zwei parallelen Strategien …
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