A Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)

Following active growth, the aquatic gram-negative rod Prolinoborus fasciculus (Aquaspirillum fasciculus) exhibited a mass conversion from its culturable rod form to a nonculturable coccoid form. Chloramphenicol did not prevent the conversion. Attempts to obtain variants that would not convert to the coccoid form were unsuccessful. Although the coccoid form fluoresced with acridine orange, agarose gel electrophoresis revealed extensive ribosomal RNA degradation. Poly-Ã -hydroxybutyrate, abundant in the vegetative rods, was not detectable in the coccoid cells. The results suggest that the coccoid form of A. fasciculus is a degenerative form rather than part of a life cycle.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biology

Department dc:contributor.department

Biology

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1998

Author dc:creator

• Koechlein, David Jacob

Chair dc:contributor.committeechair

• Krieg, Noel R.

Committee members dc:contributor.committeemember

• Yousten, Allan A.
• Claus, George William

• RNA
• acridine orange
• Viable but nonculturable
• coccoid bodies
• VBNC

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en

Dc Identifier Other

etd-110798-012740

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/35624