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Showing 1 to 10 of 10 for “"VBNC"”.
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Survival and recovery of Escherichia coli O157:H7 during starvation at sub-optimal temperatures
… entrance into the viable but non-culturable (VBNC) state where cell viability can only be detected using culture-independent methods with the number of colonies recovered decreasing over the duration of the starvation period. Findings from this study showed that formation of a VBNC …
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Limitations to Use Copper as an Antimicrobial Control of Legionella in Potable Water Plumbing Systems
… the possibility that viable but non-culturable (VBNC) Legionella might still cause human disease, were also identified as major confounding factors. These knowledge gaps are addressed from various dimensions across each chapter of the dissertation. The effects of pH, orthophosphate corrosion …
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Development of a Flow Cytometry Method for the Identification and Enumeration of Enterococcus faecalis
… cells that are in viable-but-non-culturable (VBNC) state. Initial field test using natural seawater samples spiked with <em>E. faecalis</em>, demonstrated high accuracy (98-120 % recovery rate) and precision (6-10% CV) of the method to detect <em>E. faecalis</em>. The short analysis time (~ 2 …
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Molekularbiologische Studien zur Pathogenität und Ökologie von Legionella pneumophila
… Ruhestadium (viable but nonculturable, VBNC) eintreten kann. Parallel zur Ermittlung der CFU-Werte wurden während des Verlaufs der Experimente die Lebendzellzahlen durch Fluoreszenzfärbungen bestimmt. Der Übergang von L. pneumophila in das VBNC-Ruhestadium wurde zudem durch in situ - …
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Improved Detection Methods for Selected Vibrio Species in the Marine Aquatic Environment
… with other methods, allows enumeration of the VBNC portion of the population. The work presented in this thesis serves to further characterize these bacteria and presents novel methods for their detection that may be used to mitigate their risk to human health.</p>
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The Universal Stress Proteins of Micrococcus Luteus Are Metabolic Regulators That Confer Resistance to a Diverse Set of Stress Factors
… stress response including the entering the VBNC state.
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Fate of Antibiotic Resistance Genes During Anaerobic Digestion of Wastewater Solids
… environments. Viable but nonculturable (VBNC) states are initiated by thermal treatments, such as thermophilic digestion and possibly pasteurization, and allow the persistence of pathogen cells and any ARGs contained therein through treatment and into the receiving environment where …
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Smart microscopy methods for quantitative microbiology
… by antibiotics, while some cells which have VBNC like properties are actually persisters and are able to re-grow after removal of the antibiotic. These results challenge the literature’s clean delineation between susceptible, persister, and viable but non-culturable cells. This work advances …
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A Study of the Coccoid Bodies of Prolinoboborus fasciculus (Aquaspirillum fasciculus)
Following active growth, the aquatic gram-negative rod Prolinoborus fasciculus (Aquaspirillum fasciculus) exhibited a mass conversion from its culturable rod form to a nonculturable coccoid form. Chloramphenicol did not prevent the conversion. Attempts to obtain variants that would not convert to …
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A study of the coccoid form and the autolysins of <i>Campylobacter upsaliensis</i>
Conversion of <i>Campylobacter upsaliensis</i> to the nonculturable but viable coccoid form was characterized. Chloramphenicol did not prevent the conversion. Severe decreases in isocitrate dehydrogenase activity and oxygen uptake and extensive degradation of ribosomal RNA suggest that the coccoid …