Study of the antioxidant, anti-inflammatory and anti-diabetic effects of extracts from Cotinus coggygria in models with experimental animals and cell cultures // Проучване на антиоксидантното,противовъзпалително и противодиабетно действие на екстракти от Cotinus coggygria в експериментални модели с опитни животни и клетъчни култури

[EN] The aim of the dissertation is to study the antioxidant, anti-inflammatory and anti-diabetic properties of Cotinus coggygria by: (1) Determination of the phytochemical composition and the antioxidant activity of the extracts from leaves and wood; (2) Evaluation of the toxicity of the extracts on cell cultures and experimental animals; (3) Determination of biological activity of the extracts in models of oxidative stress and inflammation. In order to resolve the aim, following tasks are performed: (1) Separation, identification and quantification of major polyphenols in extracts of leaves and wood of sumac tree by high performance liquid chromatography (HPLC); (2)Fractionation and purification of the extracts by high-performance countercurrent chromatography (HPCCC); (3) Measurements of the concentration of total polyphenols and determination of in vitro antioxidant activity of the extracts of C. coggygria; (4) MTT cytotoxicity assay of extracts of C. coggygria on cell cultures: 3T3-L1 (murine preadipocytes) and J774A.1 (murine macrophages);5)treatment of male Wistar rats with three aqueous extract from the leaves and aqueous-ethanolic extract of wood in a model of subchronic toxicity (30 days); (6) Study of in vivo antioxidant properties of the extracts from sumac tree in models of indomethacin-induced gastric ulcerogenesis and acetaminophen-induced hepatotoxicity in rats; (7) Evaluation of anti-inflammatory activities of the extracts of the sumac tree in a model of carrageenan-induced inflammation of the hind paw in rats; (8) Examination of antidiabetic potential of Cotinus coggygria by analyzing the expression of genes for adiponectin and PPARγ in murine 3T3-L1 preadipocytes.

Authors dc:creator

• Pavlov, Danail
• Павлов, Данаил

• Cotinus coggygria
• polyphenol
• antioxidant
• anti-inflammatory
• anti-diabetic
• Биохимия, молекулна медицина и нутригеномика​ / Biochemistry, Molecular Medicine, Nutrigenomics

Repository record dc:identifier

http://repository.mu-varna.bg/handle/nls/54

OAI identifier oai:identifier

oai:repository.mu-varna.bg:nls/54