The effects of acute high- and low-intensity exercise on Hsp70 and Hsp90 accumulation in rat skeletal myofibres and vasculature

Exercise induces cytoprotective stress proteins in blood vessels (BV) and skeletal myofibres. The localization and extent of induction with different exercise intensities is unclear. Rats (n=10 per group) were run at high (HIEX; 30m/min) versus low-intensity exercise (LOEX; 15m/min) for 1hr, sacrificed 24hr later. Sections of the white portion of the vastus were immunofluorescently stained for Hsp70 and Hsp90. It was hypothesized that: 1) a greater abundance of Hsp70 and Hsp90 would be observed in BVs following HIEX, 2) due to earlier recruitment, larger BVs would have a more robust response than small BVs, 3) more myofibres surrounding BVs expressing Hsp70 would express Hsp70. Ratio of BVs with Hsp70/total vessels and Hsp70 density was greater in HIEX versus LOEX (p

Name thesis:degree_name

M Sc

Discipline thesis:degree_discipline

Kinesiology

Grantor dc:publisher

The University of Western Ontario

Year dc:date.issued

2013

Author dc:creator

• Kowalchuk, Hana

Advisor dc:contributor.advisor

• Earl Noble

• Heat Shock Protein
• Hsp70
• Hsp90
• vasculature
• skeletal myofibre
• intensity-dependent
• immunofluorescence

Language dc:language.iso

en_ca

Handle dc:identifier.uri

https://hdl.handle.net/20.500.14721/36563

OAI identifier oai:identifier

oai:uwo.scholaris.ca:20.500.14721/36563