Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 521 for “"immunofluorescence"”.
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Using immunofluorescence techniques to Identify T cells in the foreskin tissue after medical male circumcision
… to the epithelium of the foreskin tissue using immunofluorescence imaging. 2) to compare manual counting and semi-automated method for counting dually positive cells. 3) to use multiparameter flow cytometry to characterize the cells recruited under the influence of CCL27. Methodology: Inner …
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Immunofluorescence of avian infectious bronchitis virus and Newcastle disease virus in singly and dually infected cell cultures
Thesis (M.S.)--Michigan State University. Dept. of Microbiology and Public Health, 1969
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Purification of SIMPL Antibody and Immunofluorescence of SIMPL Sub-Cellular Localization in Response to TNFα- and IL-1
… Western blotting. The antibody was utilized in immunofluorescence experiments on mouse endothelial cells that were either unstimulated or were stimulated (IL-1 or TNF-α). In the absence of cytokine, SIMPL was localized in both the cytoplasm and the nucleus as opposed to NF-κB which was almost …
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The use of collagen IV immunohistochemistry in the diagnosis of bullous pemphigoid
… that require histopathological correlation. Immunofluorescence is the most routinely used reliable investigation for diagnosis but requires specialised equipment and is technically sophisticated. Collagen IV immunohistochemistry is reported as a reliable test for the diagnosis of …
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Immunohistochemistry for studying cyclic nucleotides in the central nervous system, with particular reference to guanosine 3',5'-monophosphate
… the criteria of specificity for RIA, but not for immunofluorescence. 'In vivo' and 'in vitro' biochemical techniques, coupled with cyclic GMP immunofluorescence, failed to localize semi -quantitative changes in intensity and /or distribution of staining, under conditions where total nucleotide …
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Studies of proteins in the posterior lobe of the pituitary gland
… animal, reacted positively with the antiserum. Immunofluorescence histochemistry was used to demonstrate neurophysin-like antigen in tissue slices. Initially tissue was frozen unfixed and sectioned in a cryostat prior to fixation in 95% (v/v) aqueous ethanol. subsequently an improved method was …
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Lymphocyte populations and their relationship to cell-mediated immune responses in aflatoxin-treated guinea pigs
… aflatoxin-treated guinea pigs were counted using immunofluorescence and bacterial markers. No changes occurred in absolute number of B or T lymphocytes identified by immunofluorescence. The number of S schottmulleri-positive lymphocytes closely approximated the numbr of T lymphocytes identified by …
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Investigation of the expression of DMC1, a meiotic gene, in trichomonas vaginalis
… studies were done in T. vaginalis using immunofluorescence against Dmc1 protein with an HA tag. These studies showed that recombinant Dmc1 remained in the cytoplasm upon treatment with DNA damaging drugs. Additionally, T.vaginalis Dmc1 protein was expressed and purified from E.coli to …
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The structural and functional changes of blood vessels during aging
… protein expression was determined by indirect immunofluorescence. The thickness of the adventitia increased dramatically with age. Immunofluorescence revealed a robust expression of endothelin system proteins in the adventitia. Additionally, extracellular matrix proteins collagen and …
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Characterization of the Biological Functions of Human Recombinant Zona Pellucida Protein 3
… of rhZP3 was detected by hemizona assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = …
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Pathological changes in mesostriatal neurons in a PKC-gamma mutant rat
… in the midbrain cell groups using quantitative immunofluorescence microscopy of cell bodies or their surrounding neuropil (a) those molecules giving information about the capacity of midbrain aminergic cell bodies to synthesis transmitters; tyrosine hydroxylase (TH) in the dopaminergic neurons …
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ΑΝΤΙΣΩΜΑΤΑ ΚΑΤΑ ΔΕΟΞΥΡΙΒΟΝΟΥΚΛΕΙΚΟΥ ΟΞΕΟΣ (DNA). ΑΝΑΠΤΥΞΗ ΚΑΙ ΣΥΓΚΡΙΣΗ ΜΕΘΟΔΩΝ ΠΡΟΣΔΙΟΡΙΣΜΟΥ, ΚΛΙΝΙΚΗ ΣΗΜΑΣΙΑ
… ELISA WAS COMPARED WITH THE FARR ASSAY AND IMMUNOFLUORESCENCE. IT WAS DEMONSTRATED THAT ELISA IS THE MOST SENSITIVE METHOD, WITHOUT LOOSING SUBSTANTIALLY ITS SPECIFICITY, WHILE THE IMMUNOFLUORESCENCE IS THE MOST SPECIFIC, METHOD. WHEN THE SLE SERA WERE ANALYZED ACCORDING TO DISEASE ACTIVITY, …
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Evaluating convolutional neural networks and transformer architectures for image-based prediction of protein localization in eukaryotic cells
… to tackle this problem by leveraging large-scale immunofluorescence microscopy datasets. The aim of this study is to comparatively evaluate convolutional neural network (CNN) architectures and Transformer- based models for the multi-label classification of protein subcellular localization in …
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Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes
… percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
… between UL33 and UL28 was demonstrated in immunofluorescence and immunoprecipitation assays. Although all sixteen mutants appeared to interact with UL28 in co-immunoprecipitation experiments, four of the insertion mutants were defective in co-localisation with UL28 in immunofluorescence …
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Multi-modal molecular imaging of radiation therapy response in the tumour vascular microenvironment
… spatial biology modality, hyperplex sequential immunofluorescence, is applied and validated for breast cancer models vascular phenotyping, focusing on the development of a deep learning-enhanced semi-quantitative image analysis framework; iii) a preclinical radiation therapy trial is conducted …
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Identification and Characterization of the DNA Damage Response Function of Human Rif1 and a Search For Human Rap1 Interacting Factors
… 1, a telomere length regulator was identified. Immunofluorescence did not reveal an association of human Rifl with telomeres. Preliminary co-immunoprecipitation with known telomeric proteins and chromatin immunoprecipitation failed to show an interaction of human Rifl with telomeric protein …
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Lyme disease in Indiana : isolation of Borrelia burgdorferi-like organisms from Dermacentor variabilis
… of Lyme disease (LD), using dark-field and immunofluorescence microscopy, and tissue samples cultured in BSK II medium for 7 to 21 days. Spirochetes were isolated in the BSK II medium from pools of midgut diverticula from 6 D. variabilis from Martin County and pools of midgut diverticula …
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