ΑΝΤΙΣΩΜΑΤΑ ΚΑΤΑ ΔΕΟΞΥΡΙΒΟΝΟΥΚΛΕΙΚΟΥ ΟΞΕΟΣ (DNA). ΑΝΑΠΤΥΞΗ ΚΑΙ ΣΥΓΚΡΙΣΗ ΜΕΘΟΔΩΝ ΠΡΟΣΔΙΟΡΙΣΜΟΥ, ΚΛΙΝΙΚΗ ΣΗΜΑΣΙΑ

A SENSITIVE, SPECIFIC AND SIMPLE SOLID PHASE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), WAS DEVELOPED FOR THE MEASUREMENT OF IGG AND IGM ANTIBODIES TO DS DNA IN 224 OF SERA OF PATIENTS WITH AUTOIMMUNE RHEUMATIC DISEASES AND 119 SERA OF HEALTHY BLOOD DONORS. THE PRESENCE OF BOTH IGG AND IGM ANTI DS DNA WAS HIGHLY CORRELATED WITH SLE. IN ADDITION SERUM COMPLEMENT C3 AND C4 LEVELS CORRELATED NEGATIVELY WITH ANTI DS DNA LEVELS. FINALLY IN SEQUENTIAL SERA FROM 5 SLE PATIENTSTHE ANTI DS DNA ACTIVITY PROVED TO BE A RELATIVELY SENSITIVE MARKER OF SLE ACTIVITY. ELISA WAS COMPARED WITH THE FARR ASSAY AND IMMUNOFLUORESCENCE. IT WAS DEMONSTRATED THAT ELISA IS THE MOST SENSITIVE METHOD, WITHOUT LOOSING SUBSTANTIALLY ITS SPECIFICITY, WHILE THE IMMUNOFLUORESCENCE IS THE MOST SPECIFIC, METHOD. WHEN THE SLE SERA WERE ANALYZED ACCORDING TO DISEASE ACTIVITY, IT WAS SHOWN THAT ELISA AND FARR ASSAY WERE CORRELATED WELL WITH THE LUPUS ACTIVITY INDEX (LAI). ELISA ANTI DS DNA VALUES, HOWEVER, CORRELATE WITH THE CLINICAL ACTIVITY OF LUPUS, INDEPENDENTLY OF THE ANTI DS DNA LEVELS IN THE SERA.

Grantor dc:publisher

University of Ioannina

Year dc:date

1989

Authors dc:creator

• Tzioufas, Athanasios
• Τζιούφας, Αθανάσιος

• ΑΝΟΣΟΕΝΖΥΜΑΤΙΚΗ ΜΕΘΟΔΟΣ ΠΡΟΣΔΙΟΡΙΣΜΟΥ
• ΑΝΤΙΣΩΜΑΤΑ ΚΑΤΑ DS DNA
• Αυτοάνοσα νοσήματα
• ΚΛΙΝΙΚΗ ΕΝΕΡΓΟΤΗΤΑ ΤΟΥ ΣΕΛ
• ΣΥΓΚΡΙΣΗ ΜΕΘΟΔΩΝ ΠΡΟΣΔΙΟΡΙΣΜΟΥ
• Συστηματικός ερυθηματώδης λύκος ( ΣΕΛ)
• ANTI-DS DNA ANTIBODIES
• Autoimmune diseases
• ELISA
• SLE DISEASE ACTIVITY
• SYSTEMATIC LUPUS ERYTHEMATOSUS (SLE)
• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Medical and Health Sciences
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1209

OAI identifier oai:identifier

oai:10442/1209