Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes

This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

1979

Author dc:creator

• Vanlandingham, Laura Lynn

Contributors dc:contributor

• Richard Myers

• Biology

Statement dc:rights

• © Laura Lynn Vanlandingham

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1002

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2003