{"id":{"repo_id":"mo-state","oai_identifier":"oai:bearworks.missouristate.edu:theses-2003"},"canonical_url":"https://search.dev.ndltd.org/etd/mo-state/oai:bearworks.missouristate.edu:theses-2003","repository":{"repo_id":"mo-state","name":"Missouri State University","base_url":"https://bearworks.missouristate.edu/do/oai/"},"display":{"title":"Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes","abstract":"This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell.","abstract_html":"This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell.","abstract_has_math":false,"creators":["Vanlandingham, Laura Lynn"],"institution":null,"degree_name":"Master of Science in Biology","degree_level":"Masters","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Richard Myers"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1979,"date_issued":"1979-07-01T07:00:00Z","date_published":"1979-07-01T07:00:00Z","updated_at":"2026-07-24T03:16:06Z","subjects":["Biology"],"languages":[],"rights":["© Laura Lynn Vanlandingham"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://bearworks.missouristate.edu/theses/1002","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Richard Myers"]},{"key":"dc:creator","label":"Author","values":["Vanlandingham, Laura Lynn"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Masters"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Biology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["© Laura Lynn Vanlandingham"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://bearworks.missouristate.edu/theses/1002"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell."]},{"key":"dc:title","label":"Title","values":["Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes"]}]}],"canonical_facts":{"dc:contributor":["Richard Myers"],"dc:creator":["Vanlandingham, Laura Lynn"],"dc:description.abstract":["This study determined the effect of immunization with Listeria monocytogenes upon spontaneous rosette formation (SRF) between BALB/c mouse spleen lymphocytes and C57BL mouse erythrocytes. It was found that SRF in nonimmunized mice ranged from 8 to 20 percent with a mean value of 12.0 + 2.1 percent. Immunofluorescence studies indicated that 40.7 ± 7.65 percent of these rosette forming cells (RFC) were immunoglobulin G (IgG) positive. Alternatively, BALB/c mice immunized with Listeria exhibited a depressed SRF. In these mice SRF ranged from 6 to 13.5 percent with a mean value of 8.7 + 1.4 percent. In this case immunofluorescence revealed that 62.7 ± 6.0 percent of the RFCs were IgG positive. The difference between the mean SRF values in nonimmunized and immunized mice, and the difference between the mean values of IgG positive RFCs in the two systems were determined to be significant by the Student's t test. Concanavalin A (Con A) binding studies were also performed to assess differences between lymphocyte surfaces in nonimmunized and immunized mice. It is suggested that depression of SRF in immunized mice is due to a surface modulation of the murine T cell."],"dc:identifier":["https://bearworks.missouristate.edu/theses/1002"],"dc:rights":["© Laura Lynn Vanlandingham"],"dc:subject":["Biology"],"dc:title":["Spontaneous Rosette Formation In Mice Immunized With Listeria Monocytogenes"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Masters"],"thesis:degree_name":["Master of Science in Biology"]},"updated_at":"2026-07-24T03:16:06Z"}