Detection and Quantification of Cells using Magnetic Particle Imaging and Magnetic Microspheres

Magnetic particle imaging (MPI) is an emerging imaging modality that specifically detects superparamagnetic iron oxide nanoparticles (SPIOs). Our lab has shown that cell tracking with magnetic resonance imaging (MRI) has very high sensitivity, but low specificity and quantification of iron labeled cells is difficult. MPI cell tracking could overcome these limitations. A MomentumTM MPI system was installed at Robarts in August 2019 and this is the first project to be completed using MPI. In Chapter 2 a series of in vitro experiments are reported which tested the repeatability and reproducibility of imaging SPIO labeled cell samples. There are no reports of the use of micron-sized iron oxide particles (MPIO) for cell tracking by MPI. Therefore, in Chapter 3, MPIO was evaluated for in vivo detection and quantification of cancer cells in the mouse brain by MPI. In Chapter 4, limitations of these studies and plans for future work are discussed.

Name thesis:degree_name

M Sc

Discipline thesis:degree_discipline

Medical Biophysics

Grantor dc:publisher

The University of Western Ontario

Year dc:date.issued

2020

Author dc:creator

• Melo, Kierstin P

Advisor dc:contributor.advisor

• Foster, Paula

• Magnetic Particle Imaging (MPI)
• Superparamagnetic Iron Oxide (SPIO)
• Micron-Sized Iron Oxide Particles (MPIO)

Language dc:language.iso

en_ca

Handle dc:identifier.uri

https://hdl.handle.net/20.500.14721/30707

OAI identifier oai:identifier

oai:uwo.scholaris.ca:20.500.14721/30707