{"id":{"repo_id":"uwo","oai_identifier":"oai:uwo.scholaris.ca:20.500.14721/30707"},"canonical_url":"https://search.dev.ndltd.org/etd/uwo/oai:uwo.scholaris.ca:20.500.14721/30707","repository":{"repo_id":"uwo","name":"Western University","base_url":"https://uwo.scholaris.ca/server/oai/request"},"display":{"title":"Detection and Quantification of Cells using Magnetic Particle Imaging and Magnetic Microspheres","abstract":"Magnetic particle imaging (MPI) is an emerging imaging modality that specifically detects superparamagnetic iron oxide nanoparticles (SPIOs). Our lab has shown that cell tracking with magnetic resonance imaging (MRI) has very high sensitivity, but low specificity and quantification of iron labeled cells is difficult. MPI cell tracking could overcome these limitations. A MomentumTM MPI system was installed at Robarts in August 2019 and this is the first project to be completed using MPI. In Chapter 2 a series of in vitro experiments are reported which tested the repeatability and reproducibility of imaging SPIO labeled cell samples. There are no reports of the use of micron-sized iron oxide particles (MPIO) for cell tracking by MPI. Therefore, in Chapter 3, MPIO was evaluated for in vivo detection and quantification of cancer cells in the mouse brain by MPI. In Chapter 4, limitations of these studies and plans for future work are discussed.","abstract_html":"Magnetic particle imaging (MPI) is an emerging imaging modality that specifically detects superparamagnetic iron oxide nanoparticles (SPIOs). Our lab has shown that cell tracking with magnetic resonance imaging (MRI) has very high sensitivity, but low specificity and quantification of iron labeled cells is difficult. MPI cell tracking could overcome these limitations. A MomentumTM MPI system was installed at Robarts in August 2019 and this is the first project to be completed using MPI. In Chapter 2 a series of in vitro experiments are reported which tested the repeatability and reproducibility of imaging SPIO labeled cell samples. There are no reports of the use of micron-sized iron oxide particles (MPIO) for cell tracking by MPI. Therefore, in Chapter 3, MPIO was evaluated for in vivo detection and quantification of cancer cells in the mouse brain by MPI. In Chapter 4, limitations of these studies and plans for future work are discussed.","abstract_has_math":false,"creators":["Melo, Kierstin P"],"institution":"The University of Western Ontario","degree_name":"M Sc","degree_level":null,"degree_discipline":"Medical Biophysics","degree_department":null,"school":null,"contributors":[],"advisors":["Foster, Paula"],"committee_chairs":[],"committee_members":[],"year":2020,"date_issued":"2020-12-04","date_published":"2020-12-04","updated_at":"2026-07-27T21:55:58Z","subjects":["Magnetic Particle Imaging (MPI)","Superparamagnetic Iron Oxide (SPIO)","Micron-Sized Iron Oxide Particles (MPIO)"],"languages":["en_ca"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/20.500.14721/30707","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.advisor","label":"Advisor","values":["Foster, Paula"]},{"key":"dc:creator","label":"Author","values":["Melo, Kierstin P"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2025-07-10T18:44:06Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2025-07-10T18:44:06Z"]},{"key":"dc:date.issued","label":"Date","values":["2020-12-04"]},{"key":"dc:publisher","label":"Institution","values":["The University of Western Ontario"]},{"key":"dc:type","label":"Dc Type","values":["thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Medical Biophysics"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M Sc"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Magnetic Particle Imaging (MPI)","Superparamagnetic Iron Oxide (SPIO)","Micron-Sized Iron Oxide Particles (MPIO)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_ca"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/20.500.14721/30707"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."]},{"key":"dc:description.abstract","label":"Abstract","values":["Magnetic particle imaging (MPI) is an emerging imaging modality that specifically detects superparamagnetic iron oxide nanoparticles (SPIOs). Our lab has shown that cell tracking with magnetic resonance imaging (MRI) has very high sensitivity, but low specificity and quantification of iron labeled cells is difficult. MPI cell tracking could overcome these limitations. A MomentumTM MPI system was installed at Robarts in August 2019 and this is the first project to be completed using MPI. In Chapter 2 a series of in vitro experiments are reported which tested the repeatability and reproducibility of imaging SPIO labeled cell samples. There are no reports of the use of micron-sized iron oxide particles (MPIO) for cell tracking by MPI. Therefore, in Chapter 3, MPIO was evaluated for in vivo detection and quantification of cancer cells in the mouse brain by MPI. In Chapter 4, limitations of these studies and plans for future work are discussed."]},{"key":"dc:title","label":"Title","values":["Detection and Quantification of Cells using Magnetic Particle Imaging and Magnetic Microspheres"]}]}],"canonical_facts":{"dc:contributor.advisor":["Foster, Paula"],"dc:creator":["Melo, Kierstin P"],"dc:date.accessioned":["2025-07-10T18:44:06Z"],"dc:date.available":["2025-07-10T18:44:06Z"],"dc:date.issued":["2020-12-04"],"dc:description":["The thesis cover page in the PDF document includes references to Western University’s previous institutional repository platform, known as Scholarship@Western, and links to that platform (beginning with ir.lib.uwo.ca). In citing or referring to this thesis, use the DOI or handle from this page instead. Sample citation: Author name, \"Thesis title.\" (Year). Western University Open Repository. https://doi.org/10.71858/123456."],"dc:description.abstract":["Magnetic particle imaging (MPI) is an emerging imaging modality that specifically detects superparamagnetic iron oxide nanoparticles (SPIOs). Our lab has shown that cell tracking with magnetic resonance imaging (MRI) has very high sensitivity, but low specificity and quantification of iron labeled cells is difficult. MPI cell tracking could overcome these limitations. A MomentumTM MPI system was installed at Robarts in August 2019 and this is the first project to be completed using MPI. In Chapter 2 a series of in vitro experiments are reported which tested the repeatability and reproducibility of imaging SPIO labeled cell samples. There are no reports of the use of micron-sized iron oxide particles (MPIO) for cell tracking by MPI. Therefore, in Chapter 3, MPIO was evaluated for in vivo detection and quantification of cancer cells in the mouse brain by MPI. In Chapter 4, limitations of these studies and plans for future work are discussed."],"dc:identifier.uri":["https://hdl.handle.net/20.500.14721/30707"],"dc:language.iso":["en_ca"],"dc:publisher":["The University of Western Ontario"],"dc:subject":["Magnetic Particle Imaging (MPI)","Superparamagnetic Iron Oxide (SPIO)","Micron-Sized Iron Oxide Particles (MPIO)"],"dc:title":["Detection and Quantification of Cells using Magnetic Particle Imaging and Magnetic Microspheres"],"dc:type":["thesis"],"thesis:degree_discipline":["Medical Biophysics"],"thesis:degree_name":["M Sc"]},"updated_at":"2026-07-27T21:55:58Z"}