Determining biological roles of four unique Vernicia fordii acyl-CoA Binding Proteins

High-value industrial oils are essential for many processes and have great economic and environmental impacts. The tung tree produces a high-value seed oil. Approximately 80% of tung oil is α-eleostearic acid, which has a high degree of unsaturation thus giving it properties as a drying oil. The identification of the biological components in tung is imperative to further the knowledge of its processes. Four unique tung acyl-CoA binding proteins, VfACBP3a, VfACBP3b, VfACBP4, and VfACBP6 were identified and the genes encoding them were cloned and analyzed to determine their biological roles. The VfACBPs were observed to be similar to other organisms' ACBPs, especially Arabidopsis thaliana. In addition, each gene was expressed in all tung tissues. They were shown to interact with VfDGAT1 and VfDGAT2, two known components of tung lipid metabolism. Finally, VfACBP3a and VfACBP6 were expressed in the seeds of transgenic plants to study the effects of VfACBP expression on seed lipid fatty acid content.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biological Sciences

Year

2011

Author dc:creator

• Pastor, Steven

Contributors dc:contributor

• Schluchter, Wendy
• Shockey, Jay
• Clancy, Mary J.

• acyl-CoA binding protein
• Vernicia fordii
• Arabidopsis thaliana
• lipid biosynthesis
• lipid metabolism
• eleostearic acid
• tung
• Kennedy pathway
• biofuel
• industrial feedstocks
• qPCR
• split ubiquitin

Repository record dc:identifier

https://scholarworks.uno.edu/td/1337

OAI identifier oai:identifier

oai:scholarworks.uno.edu:td-2321