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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 768 for “"qPCR"”.

  1. DYNAMICS OF MICROCYSTIS-INFECTING CYANOPHAGES IN TROPICAL FRESHWATERS THROUGH QPCR AND NEXT GENERATION SEQUENCING

    LOH LAY LENG2017

    Cyanophages (i.e. viruses that infect cyanobacteria) are crucial in regulating the abundance of their host cells, affecting the clonal diversity as well as the composition of the cyanobacterial community. This thesis focuses on two environmental water studies based on short- (72-hour diel cycle) …

    nus Repository record

  2. Investigating the distribution, seasonal dynamics and toxicity of Azadinium spinosum in Scottish waters using qPCR

    Paterson, RuthUniversity of Edinburgh2018

    … realtime<br/>polymerase chain reaction (qPCR) is discussed in detail and documents its first use in Scottish waters to survey A. spinosum abundance and seasonality. The limit of detection of the method was found to be 2000 ±5600 cells L-1, however it is unclear whether this is adequate …

    uhi-uk Repository record

  3. Assessment of Microbial Biodegradation of Mixed Soil Contaminants at the Santa Susan Field Laboratory Using TRFLP, qPCR, and Culturing

    Croyle, Kenny William2014

    … (TRFLP), quantitative polymerase chain reaction (qPCR), and traditional culturing techniques combined with sequencing of the 16S or ITS regions of the cultured bacteria and fungi. Soil was collected from the Santa Susana Field Laboratory (SSFL), which was the site of numerous liquid-propulsion …

    calpoly Repository record

  4. Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome

    Jackson, Tamra A.University of Illinois at Urbana-Champaign2015

    … quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in 14.5% of the samples …

    uiuc Repository record

  5. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation

    Tyler, Ronald Dale Jr.Virginia Tech2012

    … quantitative polymerase chain reaction (qPCR) assay and had a sensitivity of 92%. To determine assay specificity, 21 FFPE tissues from animals without gastrointestinal disease combined with 13 FFPE tissues from non-ruminant animals (frog, dove, turtle, dog, and 2 cats) with …

    vt Repository record

  6. Accelerated Sepsis Diagnosis by Seamless Integration of Nucleic Acid Purification and Detection

    Hsu, Bang-Ning2014

    … diagnostic information. Although the present qPCR-based test is more specific than biomarker detection and faster than culturing, its 6 ~ 10 hr turnaround remains suboptimal relative to the 7.6%/hr rapid deterioration of the survival rate, and the 3 hr hands-on time is labor-intensive. To …

    duke Repository record

  7. NLRP2 expressziójának vizsgálata különböző immortalizált sejtvonalakon

    Bodrogi, Boglárka2022

    … A kezelt sejtekből RNS-t izoláltunk, majd RT-qPCR segítségével meghatároztuk az NLRP2 relatív expresszióját. Az eredményeket ciklofilinnel normalizáltuk. A vizsgált sejtvonalak közül a THP-1, K562, Caco2 és A549 sejteken detektáltuk az NLRP2 jelenlétét, viszont az NLRP2 expresszióját …

    debrecen Repository record

  8. Development and clinical performance of nucleic acid amplification techniques for the diagnosis of Strongyloides stercoralis

    Bowers, K.University of Westminster2017

    … for this study were a published real- time PCR (qPCR) assay (ten Hove et al., 2009) and a novel LAMP assay. The NAATs were compared to the combined reference standard of microscopy, culture and serology for the diagnosis of S. stercoralis in stool samples. The development of the novel LAMP assay …

    westminster Repository record

  9. Repair of DNA-protein crosslinks in mammalian cells

    Chesner, Lisa2018

    … polymerase chain reaction (SSPE-qPCR) used to study the repair of DNA-protein crosslinks in mammalian cells. DNA-protein crosslinks (DPCs) are bulky lesions which disrupt important cell processes such as transcription and replication. They are formed by endogenous molecules such …

    umn Repository record

  10. Evaluations of Diagnostic Tests for Undifferentiated Febrile Illness on the Thailand-Myanmar (Burma) Border

    Watthanaworawit, WanitdaThe Open University2018

    … IgM ICT and 47kDa quantitative real-time PCR (qPCR) for scrub typhus and 17kDa qPCR for murine typhus were evaluated using acute plasma specimens for the IgM ICT and acute buffy coat specimens for the qPCRs. Sensitivities of the IgM ICT, 47kDa qPCR and 17kDa qPCR were 27.3% (95% CI 15.0-42.8), …

    the-open-u Repository record

  11. Expression levels of non-autonomous retrotransposons in germ-line rodent tissues

    Wiesner, Catherine Elaine2010

    … mobility and vertical transmission. RT-PCR and qPCR results suggested higher expression of both SINE and LINE elements in germ-line tissues over somatic. Additionally, the qPCR findings demonstrated higher expression of the IDL element by at least one order of magnitude than the guinea pig ID or …

    emich Repository record

  12. Determination of soybean cultivar resistance to soybean cyst nematode with quantitative polymerase chain reaction

    Lopez Nicora, Horacio D.University of Illinois at Urbana-Champaign2010

    … quantitative Polymerase Chain Reaction (qPCR), a prelude to differentiation of resistance levels in soybean cultivars. Two experiments were conducted. In the first one, a consistent inoculation method was developed using to provide active second-stage juveniles (J2). Two-day-old soybean …

    uiuc Repository record

  13. Tongue dorsum swab processing for the detection of tuberculosis with Cepheid Xpert® MTB/RIF Ultra

    Whitman, Grant Russell2021

    … from studies that used manual quantitative PCR (qPCR) to analyze swabs. Studies using the automated Cepheid Xpert® MTB/RIF Ultra qPCR test (Xpert Ultra) to analyze tongue swabs have yielded more modest results. Using tongue swabs collected from TB-negative volunteers and spiked with cultured MTB …

    washington Repository record

  14. An experimental investigation into the mechanisms of bacterial evolution

    Wan, ZhenmaoThe Graduate School and University Center of The City University of New York2001

    … the quantitative polymerase chain reaction (qPCR) to provide real-time enumeration of genetic marker abundance within bacterial populations. Second, I will focus on horizontal gene transfer in <em>E. coli</em> occurring through a process called conjugation. By fitting the qPCR data to a …

    cuny-grad Repository record

  15. Microbial Source Tracking of Bacteroides to Identify Human and Bovine Fecal Loading in McDaniel Lake

    Somerville, Kalie Michelle

    … fecal sources. Using quantitative real-time PCR (qPCR), human-associated and bovine-associated Bacteroides DNA, as well as total Bacteroides DNA can be quantified and concentrations of fecal contamination in lakes and streams can be determined. Amplification control plasmids containing 16S rDNA …

    mo-state Repository record

  16. Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health

    Libert, XavierULiège - Université de Liège2017

    … a specific ITS-based SYBR®green real-time PCR (qPCR) assay for Aspergillus versicolor, a species frequently observed in indoor air and known to be allergenic. Additionally, an ITS-based qPCR assay was developed for the specific detection of Exophiala jeanselmei, a pathogenic yeast suspected to …

    liege Repository record

  17. Optimization of automated immunomagnetic separation for the detection of escherichia coli 0157: H7 by quantitative polymerase chain reaction

    Khan, AyeshaUniversity of Central Lancashire2006

    … such as quantitative polymerase chain reaction (qPCR) assays have been developed, which are specific, quick and reliable. The major drawback of the technique is prior DNA extraction, which can be time consuming and result in partial loss of target material, followed by small sample input into the …

    cent-lancashire Repository record

  18. Characterizing the epidemiology of ophidiomycosis in North American snakes through field studies, modeling, pathogen genomic analysis, and treatment trials

    Haynes, EllenUniversity of Illinois at Urbana-Champaign2021

    … to detect the presence of O. ophidiicola using qPCR. Each snake was assigned to an ophidiomycosis category based on the presence of skin lesions and O. ophidiicola. Logistic regression models were used to predict lesion presence, O. ophidiicola detection, and ophidiomycosis category based on …

    uiuc Repository record

  19. Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization

    Sayre, Autumn2019

    … primers were designed and tested in PCR and qPCR assays against swine and non-target DNA. Sequence A5, closely related to Prevotellaceae, was detected in all swine samples tested, as well as the environmental samples impacted by swine manure. The relationship between closely related 16S rRNA …

    wvu Repository record

  20. The identification of biomarker differences between grade I and II meningiomas: Fibulin-2 is a novel biomarker for differentiating grade II from grade I meningiomas

    Sofela, Agbolahan2021

    … (WB) and quantitative polymerase chain reaction (qPCR) in primary cells; via WB, immunohistochemistry and qPCR in tissue; and in plasma, via enzyme-linked immunosorbent assay (ELISA). Results: I identified 3554 proteins commonly expressed in both meningioma grades. Further analysis revealed that …

    plymouth Repository record

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